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Literature summary for 3.1.3.2 extracted from

  • Lau, K.H.W.; Freeman, T.K.; Baylink, D.J.
    Purification and characterization of an acid phosphatase that displays phosphotyrosyl-protein phosphatase activity from bovine cortical bone matrix (1987), J. Biol. Chem., 262, 1389-1397.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
1 * 32500, SDS-PAGE Bos taurus
34600
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the charge heterogeneity of isoenzymes might be a result of varying degrees of glycosylation Bos taurus

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate formation of a covalent phosphoryl-enzyme intermediate, kinetic pattern of a pseudo uni-bi hydrolytic two-step reaction mechanism Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
bone cortical bone matrix Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.15
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O at 12.9% of the activity with p-nitrophenyl phosphate Bos taurus AMP + phosphate
-
?
ATP + H2O at 18.0% of the activity with p-nitrophenyl phosphate Bos taurus ADP + phosphate
-
?
CDP + H2O at 6.6% of the activity with p-nitrophenyl phosphate Bos taurus CMP + phosphate
-
?
GDP + H2O at 18.5% of the activity with p-nitrophenyl phosphate Bos taurus GMP + phosphate
-
?
GTP + H2O at 15.3% of the activity with p-nitrophenyl phosphate Bos taurus GDP + phosphate
-
?
O-phospho-L-Tyr + H2O at 8.2% of the activity with p-nitrophenyl phosphate Bos taurus Tyr + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Bos taurus p-nitrophenol + phosphate
-
?
phosphotyrosyl histone + H2O 4.2% of the activity with p-nitrophenyl phosphate Bos taurus histone + phosphate
-
?
TDP + H2O at 10.6% of the activity with p-nitrophenyl phosphate Bos taurus TMP + phosphate
-
?
TTP + H2O at 11.3% of the activity with p-nitrophenyl phosphate Bos taurus TDP + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 32500, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
hydrolysis of p-nitrophenyl phosphate Bos taurus
7
-
hydrolysis of nucleoside triphosphate, nucleoside diphosphate, phosphotyrosine and phosphoryltyrosyl histone Bos taurus