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Literature summary for 3.1.3.18 extracted from

  • Pellicer, M.T.; Nunez, M.F.; Aguilar, J.; Badia, J.; Baldoma, L.
    Role of 2-phosphoglycolate phosphatase of Escherichia coli in metabolism of the 2-phosphoglycolate formed in DNA repair (2003), J. Bacteriol., 185, 5815-5821.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Cl- the activity is 11fold increased, cooperative effect of Cl- and Mg2+ Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
2-Phosphoglycolate 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- the activity is 11fold increased, cooperative effect of Cl- and Mg2+ Escherichia coli
Mg2+ cooperative effect of Cl- and Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27390
-
calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Phosphoglycolate + H2O Escherichia coli the enzyme is involved in a reaction relevant for DNA repair Glycolate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Cupriavidus necator
-
-
-
Escherichia coli
-
-
-
Escherichia coli P32662
-
-
Haemophilus influenzae
-
-
-
Klebsiella aerogenes Q9EYY5
-
-
Salmonella enterica
-
-
-
Shigella flexneri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Phosphoglycolate + H2O
-
Escherichia coli Glycolate + phosphate
-
?
2-Phosphoglycolate + H2O the enzyme is involved in a reaction relevant for DNA repair Escherichia coli Glycolate + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
94
-
2-Phosphoglycolate 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
Escherichia coli