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Literature summary for 3.1.3.17 extracted from

  • McNall, S.J.; Ballou, L.M.; Villa-Moruzzi, E.; Fischer, E.H.
    Purification and characterization of phosphorylase phosphatase from rabbit skeletal muscle (1988), Methods Enzymol., 159, 377-390.
    View publication on PubMed
Search for more literature for 3.1.3.17

Activating Compound

Activating Compound Comment Organism Structure
additional information the inactive enzyme can be activated in two ways: 1. by limited proteolysis with trypsin in presence of Mn2+, 2. activation by an MgATP2--dependent reaction, that also requires a factor known as Fa, which appears to be identical to glycogen synthase kinase-3 Oryctolagus cuniculus

General Stability

General Stability Organism
the 31000 Da regulatory subunit is extremely susceptible to proteolysis, rapidly destroyed by incubation with trypsin Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
protein heat stable proteins: inhibitor 1 and 2 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the inactive enzyme can be activated in two ways: 1. by limited proteolysis with trypsin in presence of Mn2+, 2. activation by an MgATP2--dependent reaction, that also requires a factor known as Fa, which appears to be identical to glycogen synthase kinase-3 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 31000 regulatory subunit + 1 * 38000 catalytic subunit, SDS-PAGE Oryctolagus cuniculus
38000
-
 1 * 31000 regulatory subunit + 1 * 38000 catalytic subunit, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oryctolagus cuniculus the enzyme plays an important role in carbohydrate metabolism by affecting the activity of phosphorylase, glycogen synthase and phosphorylase kinase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle skeletal Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme plays an important role in carbohydrate metabolism by affecting the activity of phosphorylase, glycogen synthase and phosphorylase kinase Oryctolagus cuniculus ?
-
 ?
phosphorylase a + H2O
-
 Oryctolagus cuniculus phosphorylase b + phosphate
-
 ?
phosphorylase kinase + H2O preferentially dephosphorylates the beta subunit over the alpha subunit Oryctolagus cuniculus phosphate + dephosphorylated phosphorylase kinase
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 31000 regulatory subunit + 1 * 38000 catalytic subunit, SDS-PAGE Oryctolagus cuniculus