Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant PPIalpha in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E42A | site-directed mutagenesis, the mutant shows only slightly reduced sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme | Homo sapiens |
E42C | site-directed mutagenesis, the mutant shows only slightly reduced sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme | Homo sapiens |
E42T | site-directed mutagenesis, the mutant shows only slightly reduced sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme | Homo sapiens |
V41D | site-directed mutagenesis, the mutant shows unaltered sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme | Homo sapiens |
W43A | site-directed mutagenesis, the mutant shows unaltered sensitivity to inhibition by Inhibitor-3 compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
human Inhibitor-3 | possesses a putative protein phosphatase-1 binding motif, 39KKVEW43. A second interaction site, whose deletion leads to loss of inhibitory potency, is identified between residues 65-77 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant PPIalpha from Escherichia coli to near homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylated phosphorylase a + H2O | - |
Homo sapiens | phosphorylase a + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | PP1 possesses a hydrophobic pocket which binds a peptide motif of the type RVXF that is present in most of the known PP1 targeting/regulatory subunits | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PPI | - |
Homo sapiens |
PPIalpha | - |
Homo sapiens |
protein phosphatase-1 | - |
Homo sapiens |