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Literature summary for 3.1.3.16 extracted from

  • Kelker, M.S.; Page, R.; Peti, W.
    Crystal structures of protein phosphatase-1 bound to nodularin-R and tautomycin: a novel scaffold for structure-based drug design of serine/threonine phosphatase inhibitors (2009), J. Mol. Biol., 385, 11-21.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells with the GroEL-ES chaperone Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with nodularin-R and tautomycin, PP1:nodularin-R is crystallized in 20% (w/v) PEG 3350, 0.2 M NaI. PP1:tautomycin is crystallized in 0.1 M Tris pH 8.0, 30% (w/v) PEG 6K, 1M LiCl Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tautomycin tautomycin preferentially binds PP1 over PP2A Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00276
-
 4-nitrophenyl phosphate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P62136
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PP1
-
 Homo sapiens
PP2A
-
 Homo sapiens
protein phosphatase 1
-
 Homo sapiens
protein phosphatase 2A
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.8
-
 4-nitrophenyl phosphate
-
 Homo sapiens