Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Inhibitor-2 | marginally inhibited by 200 nM | Tulipa gesneriana | |
okadaic acid | inhibition at 1 nM | Tulipa gesneriana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
free catalytic subunit, SDS-PAGE | Tulipa gesneriana |
65000 | - |
1 * 75000 + 1 * 65000 + 1 * 38000, SDS-PAGE | Tulipa gesneriana |
75000 | - |
1 * 75000 + 1 * 65000 + 1 * 38000, SDS-PAGE | Tulipa gesneriana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tulipa gesneriana | - |
cultivar Ile de France | - |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose CL 4B column chromatgraphy, DEAE-Toyopearl 650 column chromatography, and Microcystin-Sepharose affinity chromatography | Tulipa gesneriana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
petal | - |
Tulipa gesneriana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
cell extract, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C | Tulipa gesneriana |
380 | - |
after 623fold purification, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C | Tulipa gesneriana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Tulipa gesneriana | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 75000 + 1 * 65000 + 1 * 38000, SDS-PAGE | Tulipa gesneriana |
Synonyms | Comment | Organism |
---|---|---|
PP2A | - |
Tulipa gesneriana |
protein phosphatase 2A | - |
Tulipa gesneriana |
Ser/Thr phosphatase | - |
Tulipa gesneriana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000009 | - |
catalytic 38-kDa subunit, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C | Tulipa gesneriana | okadaic acid | |
0.00000011 | - |
holoenzyme, in 100 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 2 mM p-nitrophenyl phosphate, at 25°C | Tulipa gesneriana | okadaic acid |