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Literature summary for 3.1.3.16 extracted from

  • Suhre, M.H.; Wegele, H.; Wandinger, S.K.
    Expression, purification and refolding of the phosphatase domain of protein phosphatase 1 (Ppt1) from Saccharomyces cerevisiae (2006), Int. J. Biol. Macromol., 39, 23-28.
    View publication on PubMed
Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3 codon+ cells in unsoluble inclusion bodies Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21
-
 p-nitrophenyl phosphate purified, catalytically active 40 kDa-subunit, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C Saccharomyces cerevisiae
34
-
 p-nitrophenyl phosphate full-length Ppt1, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for activity Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 1 * 4000 + 1 * 20000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA column chromatography Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
by stepwise dilution in 700 mM Tris pH 7.5, 250 mM arginine at 4°C, at a final protein concentration of 0.1 mg/ml Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
 Saccharomyces cerevisiae p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 4000 + 1 * 20000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
PPT1
-
 Saccharomyces cerevisiae
protein phosphatase 1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 p-nitrophenyl phosphate full-length Ppt1, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C Saccharomyces cerevisiae
0.78
-
 p-nitrophenyl phosphate purified, catalytically active 40 kDa-subunit, in 300 mM Tris pH 7.8, 150 mM potassium chloride, 25 mM magnesium chloride, 5 mM dithiothreitol, at 30°C Saccharomyces cerevisiae