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Literature summary for 3.1.3.16 extracted from

  • Xie, X.J.; Xue, C.Z.; Huang, W.; Yu, D.Y.; Wei, Q.
    The beta12-beta13 loop is a key regulatory element for the activity and properties of the catalytic domain of protein phosphatase 1 and 2B (2006), Biol. Chem., 387, 1461-1467.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21(lDE3) and DH5alpha Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information mutants CNAaDELTAloop [containing the beta12-beta13 loop of PP1c in place of the L7 loop] and PP1DELTAloop [with the L7 loop (from Y311 to K318) substituted for the beta12-beta13 loop (from Y272 to A279)] show no activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activating effect at 2 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.659
-
subunit CNAa-PP1-loop, crude cell lysate Homo sapiens
0.665
-
subunit PP1-CNAa-loop, crude cell lysate Homo sapiens
7.087
-
subunit CNAa-PP1-loop, after 10.8fold purification Homo sapiens
10.77
-
subunit PP1-CNAa-loop, after 16.2fold purification Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?
myelin basic protein + H2O
-
Homo sapiens ? + phosphate
-
?
phosphorylase a + H2O
-
Homo sapiens ?
-
?
RII peptide + H2O
-
Homo sapiens ? + phosphate
-
?

Synonyms

Synonyms Comment Organism
calcineurin
-
Homo sapiens
CNAa
-
Homo sapiens
PP1
-
Homo sapiens
PP2B
-
Homo sapiens
protein phosphatase 1
-
Homo sapiens
protein phosphatase 2B
-
Homo sapiens
Ser/Thr protein phosphatase
-
Homo sapiens