Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) as wild-type and selenomethionine-labeled variants | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled enzymes with bound phosphate, hanging drop vapour diffusion method, 18°C, 10 mg/ml protein in 50 mM NaCl, 20 mM Tris, pH 7.5, 0.5 mM Tris(2-carboxyethyl)phosphine hydrochloride, and 1 mM MnCl2, mixed with equal volume of reservoir solution containing 8% PEG 10000, 0.1 M sodium cacodylate, 0.5 mM TCEP, and 25% ethylene glycol, grown crystals are soaked in 0.1 M HEPES, pH 7.5, and 25 mM 4-nitrophenyl phosphate at 25°C, X-ray diffraction structure determination and analysis at 1.95 A resolution | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzyme contains a two-metal center | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by gel filtration and ion exchange chromatography | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate | catalytic mechanism, PstPpp catalytic domain structure and active site structure, structure-function relationship | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Mycobacterium tuberculosis | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the Ser/Thr protein phosphatase PP2C-family | Mycobacterium tuberculosis |
PstP/Ppp | - |
Mycobacterium tuberculosis |
PstPpp | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |