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Literature summary for 3.1.3.16 extracted from
- PrpZ, a Salmonella enterica serovar typhi serine/threonine protein phosphatase 2C with dual substrate specificity (2005), Microbiology, 151, 1159-1167.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene prpZ, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Salmonella enterica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Salmonella enterica |  |
| additional information | no inhibition by okadaic acid | Salmonella enterica | |
| Sodium diphosphate | - |
Salmonella enterica | |
| Sodium fluoride | - |
Salmonella enterica | |
| Sodium phosphate | - |
Salmonella enterica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | very high Km for RRLIEDAEY(P)AARG above accurate detection range | Salmonella enterica | |
| 0.0026 | - |
tyrosine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
| 0.019 | - |
serine/threonine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
| 0.13 | - |
RRAT(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
| 0.24 | - |
RRAS(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
| 5.7 | - |
4-nitrophenyl phosphate | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates, 40fold less effective than Mn2+ at pH 9.5 | Salmonella enterica | |
| Mn2+ | activates, 40fold more effective than Mg2+ at pH 9.5 | Salmonella enterica | |
| additional information | no effect by Ca2+ and Zn2+, metal ion preference depends strongly on the pH value, overview | Salmonella enterica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43827 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE, x * 43827, recombinant His-tagged enzyme, mass spectrometry | Salmonella enterica |
| 51000 | - |
x * 51000, recombinant His-tagged enzyme, SDS-PAGE, x * 43827, recombinant His-tagged enzyme, mass spectrometry | Salmonella enterica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
serovar Typhi, strains CT18 and Ty2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Salmonella enterica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Salmonella enterica | 4-nitrophenol + phosphate | - |
? | |
| additional information | the enzyme preforms dephosphorylation of phosphoserine, phosphothreonine, and also phosphotyrosine residues | Salmonella enterica | ? | - |
? | |
| RRAS(P)VA + H2O | synthetic substrate | Salmonella enterica | RRASVA + phosphate | - |
? | |
| RRAT(P)VA + H2O | synthetic substrate | Salmonella enterica | RRATVA + phosphate | - |
? | |
| RRLIEDAEY(P)AARG + H2O | synthetic substrate | Salmonella enterica | RRLIEDAEYAARG + phosphate | - |
? | |
| serine/threonine-phosphorylated myelin basic protein + H2O | - |
Salmonella enterica | myelin basic protein + phosphate | - |
? | |
| tyrosine-phosphorylated myelin basic protein + H2O | - |
Salmonella enterica | myelin basic protein + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 51000, recombinant His-tagged enzyme, SDS-PAGE, x * 43827, recombinant His-tagged enzyme, mass spectrometry | Salmonella enterica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the PPM family, cf. EC 3.1.3.48, protein-tyrosine-phosphatase | Salmonella enterica |
| PP2C | - |
Salmonella enterica |
| PrpZ | - |
Salmonella enterica |
| serine/threonine protein phosphatase 2C | - |
Salmonella enterica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
tyrosine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
| 0.9 | - |
RRAS(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
| 1.1 | - |
serine/threonine-phosphorylated myelin basic protein | pH 8.0, recombinant His-tagged enzyme | Salmonella enterica | |
| 2.5 | - |
RRAT(P)VA | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
| 7.4 | - |
4-nitrophenyl phosphate | pH 9.5, recombinant His-tagged enzyme | Salmonella enterica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Salmonella enterica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.5 | - |
Salmonella enterica |
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