Cloned (Comment) | Organism |
---|---|
tetracycline-inducible, stable overexpression of FLAG-tagged PP2A subunits in HEK-293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PP2A subunit knock-down by RNAi, effects of altered subunit Balpha/delta level on cellular phosphoprotein levels, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
okadaic acid | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PP2A regulates a wide variety of cellular signal transduction pathways, and performs positive regulation of Raf1-MEK1/2-ERK1/2 signaling at multiple steps of the pathway, overview | ? | - |
? | |
phosphorylated ERK1 + H2O | Homo sapiens | - |
ERK1 + phosphate | - |
? | |
phosphorylated ERK2 + H2O | Homo sapiens | - |
ERK2 + phosphate | - |
? | |
phosphorylated Raf1 + H2O | Homo sapiens | - |
Raf-1 + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged PP2A subunits from HEK-293T cells by anti-FLAG immunoaffinity chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
reconstitution of PP2A holoenzymes with recombinant subunits, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PP2A regulates a wide variety of cellular signal transduction pathways, and performs positive regulation of Raf1-MEK1/2-ERK1/2 signaling at multiple steps of the pathway, overview | Homo sapiens | ? | - |
? | |
phosphorylated ERK1 + H2O | - |
Homo sapiens | ERK1 + phosphate | - |
? | |
phosphorylated ERK2 + H2O | - |
Homo sapiens | ERK2 + phosphate | - |
? | |
phosphorylated Raf1 + H2O | - |
Homo sapiens | Raf-1 + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PP2A | - |
Homo sapiens |
protein serine/threonine phosphatase 2A | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |