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Literature summary for 3.1.3.16 extracted from

  • Swingle, M.R.; Honkanen, R.E.; Ciszak, E.M.
    Structural basis for the catalytic activity of human serine/threonine protein phosphatase-5 (2004), J. Biol. Chem., 279, 33992-33999.
    View publication on PubMed
Search for more literature for 3.1.3.16

Cloned(Commentary)

Cloned (Comment) Organism
the PP5 catalytic domain comprising residues 169-499 is expressed as His-tagged maltose-binding protein fusion protein containing a tobacco etch virus cleavage site in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged PP5 catalytic domain, hanging drop vapour diffusion method, 22°C, 20 mg/ml protein in 40 mM Tris, pH 7.5, is mixed with an equal volume of reservoir solution containing 45-60% 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 1.6 A resolution Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ trace amounts Homo sapiens
Mn2+ 1 ion per enzyme molecule, involved in metal-mediated substrate hydrolysis Homo sapiens
Zn2+ trace amounts Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PP5 affects many signaling pathways regulating cell growth and cellular stress response ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P36873
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PP5 catalytic domain maltose-binding protein fusion protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, after cleavage of the fusion protein the His-tagged enzyme is further purified by anion exchange chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate structural basis for the catalytic activity, the catalytic motif Asp271-metal1:metal2-water1-His427-His304-Asp274 requires precise substrate positioning, catalytic mechanism, metal-mediated substrate hydrolysis Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PP5 affects many signaling pathways regulating cell growth and cellular stress response Homo sapiens ?
-
 ?
additional information the enzyme performs metal-mediated substrate hydrolysis Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PP5
-
 Homo sapiens
serine/threonine protein phosphatase-5
-
 Homo sapiens