Cloned (Comment) | Organism |
---|---|
genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1, DNA and amino acid sequence determination and analysis, expression analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
D415A | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate | Synechocystis sp. |
D415N | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate | Synechocystis sp. |
D444N | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate | Synechocystis sp. |
D604N | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate | Synechocystis sp. |
D608N | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate, the activity with phosphotyrosine-RCML is highly increased compared to the wild-type enzyme | Synechocystis sp. |
D648N | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate | Synechocystis sp. |
E414Q | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate, the activity with 4-nitropehnyl phosphate is similar to the wild-type enzyme | Synechocystis sp. |
E618Q | site-directed mutagenesis of SynPPM3, the mutant shows reduced activity compared to the wild-type enzyme, the loss of activity differs in dependence of the substrate | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
phosphoserine-casein | pH 7.5, 37°, recombinant SynPPM3 | Synechocystis sp. | |
0.00045 | - |
phosphoserine-myelin basic protein | pH 7.5, 37°, recombinant SynPPM3 | Synechocystis sp. | |
0.0017 | - |
phosphoserine-RCML | pH 7.5, 37°, recombinant SynPPP1 | Synechocystis sp. | |
0.0063 | - |
phosphoserine-myelin basic protein | pH 7.5, 37°, recombinant SynPPM3 | Synechocystis sp. | |
0.022 | - |
phosphoserine-myelin basic protein | pH 7.5, 37°, recombinant SynPPP1 | Synechocystis sp. | |
12.7 | - |
4-nitrophenyl phosphate | pH 7.5, 37°, recombinant SynPPM3 | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Synechocystis sp. | |
Mn2+ | required | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803, genes sll1033 and sll1387 encoding SynPPM3 and SynPPP1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged SynPPM3 and SynPPP1 and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Synechocystis sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate | the enzymes of Synechocystis sp. also perform the protein tyrosine phosphatase reaction, EC 3.1.3.48 | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activities of mutant SynPPM3 compared to wild-type SynPPM3 | Synechocystis sp. |
0.0001 | - |
purified recombinant SynPPM3, substrate phosphotyrosine-casein | Synechocystis sp. |
0.0007 | - |
purified recombinant SynPPM3, substrate phosphotyrosine-RCML | Synechocystis sp. |
0.0017 | - |
purified recombinant SynPPM3, substrate phosphoserine-RCML | Synechocystis sp. |
0.0017 | - |
purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein | Synechocystis sp. |
0.0029 | - |
purified recombinant SynPPP1, substrate phosphoserine-myelin basic protein | Synechocystis sp. |
0.0034 | - |
purified recombinant SynPPP1, substrate phosphoserine-casein | Synechocystis sp. |
0.0044 | - |
purified recombinant SynPPP1, substrate phosphotyrosine-casein | Synechocystis sp. |
0.0053 | - |
purified recombinant SynPPM3, substrate phosphoserine-casein | Synechocystis sp. |
0.0055 | - |
purified recombinant SynPPP1, substrate phosphotyrosine-RCML | Synechocystis sp. |
0.0121 | - |
purified recombinant SynPPP1, substrate phosphoserine-RCML | Synechocystis sp. |
0.0178 | - |
purified recombinant SynPPM3, substrate phosphoserine-myelin basic protein | Synechocystis sp. |
0.066 | - |
purified recombinant SynPPM3, substrate 4-nitrophenyl phosphate | Synechocystis sp. |
0.084 | - |
purified recombinant SynPPP1, substrate 4-nitrophenyl phosphate | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Synechocystis sp. | 4-nitrophenol + phosphate | - |
? | |
phosphoserine-casein + H2O | - |
Synechocystis sp. | casein + phosphate | - |
? | |
phosphoserine-myelin basic protein + H2O | - |
Synechocystis sp. | myelin basic protein + phosphate | - |
? | |
phosphoserine-RCML + H2O | i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphoserine | Synechocystis sp. | RCML + phosphate | - |
? | |
phosphotyrosine-casein + H2O | - |
Synechocystis sp. | casein + phosphate | - |
? | |
phosphotyrosine-myelin basic protein + H2O | - |
Synechocystis sp. | myelin basic protein + phosphate | - |
? | |
phosphotyrosine-RCML + H2O | i.e. reduced carboxyamidomethylated and maleylated lysozyme containing phosphotyrosine | Synechocystis sp. | RCML + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.48 | Synechocystis sp. |
protein sewrine/threonine phosphatase | - |
Synechocystis sp. |
SynPPM3 | - |
Synechocystis sp. |
SynPPP1 | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Synechocystis sp. |