Literature summary for 3.1.3.15 extracted from

Omi, R.; Goto, M.; Miyahara, I.; Manzoku, M.; Ebihara, A.; Hirotsu, K.
Crystal structure of monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8 (2007), Biochemistry, 46, 12618-12627.

Search for more literature for 3.1.3.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapour diffusion method with 0.68 M sodium malonate and 100 mM Tris-HCl (pH 8.5)	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.27	-	L-histidinol phosphate	-	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	part of the trinuclear metal center	Thermus thermophilus
Zn2+	part of the trinuclear metal center	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29500	-	4 * 29500, dynamic light scattering	Thermus thermophilus
29976	-	4 * 29976, calculated from sequence of cDNA	Thermus thermophilus
118000	-	dynamic light scattering	Thermus thermophilus
119900	-	calculated from sequence of cDNA	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-histidinol phosphate + H2O	-	Thermus thermophilus	L-histidinol + phosphate	-	?
L-histidinol phosphate + H2O	-	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	L-histidinol + phosphate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 29500, dynamic light scattering	Thermus thermophilus
homotetramer	4 * 29976, calculated from sequence of cDNA	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
HisPPase	-	Thermus thermophilus
histidinol phosphate phosphatase	-	Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.93	-	L-histidinol phosphate	-	Thermus thermophilus

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Release 2023.1 (January 2023)