Cloned (Comment) | Organism |
---|---|
recombinant overexpression of trehalose-6-phosphate synthase/phosphatase (TPSP) in transgenic Solanum lycopersicum cv. Joyful leading to enhanced level of trehalose in the seeds | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | trehalose has been implicated in tolerance to abiotic stress in plants, so the response to heat stress of seeds from the transgenic lines of Solanum lycopersicum cv. Joyful and seeds from wild-type plants are compared. Wild-type heat-treated seeds have a germination rate of 17%, while four transgenic lines have germination rates over 50% following heat stress at 55°C. In one heat-treated transgenic line, 100% of the seeds germinated, and therefore had a germination rate six times that of seeds from wild-type plants. Quantitative PCR reveals that the expression of diverse genes that respond to heat stress is enhanced in TPSP transgenic seeds compared to wild-type seeds 150 min after the onset of heat stress. The enhanced germination rate and expression of these genes in the transgenic seeds are essentially mimicked in wild-type seeds treated with 1 mM exogenous trehalose. Accumulated trehalose and associated metabolites may act as signaling molecules that enhance the expression of heat stress-responsive genes and confer heat-stress tolerance to seeds. Changed of transcript levels of imbibed seeds in TPSP transgenic and wild type with exogenous trehalose under no heat-treated condition, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose 6-phosphate + H2O | Escherichia coli | - |
alpha,alpha-trehalose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P31677 AND P31678 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose 6-phosphate + H2O | - |
Escherichia coli | alpha,alpha-trehalose + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPSP | - |
Escherichia coli |
trehalose-6-phosphate synthase/phosphatase | - |
Escherichia coli |