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Literature summary for 3.1.3.12 extracted from

  • Li, H.; Wu, M.; Shi, Y.; Javid, B.
    Over-expression of the mycobacterial trehalose-phosphate phosphatase OtsB2 results in a defect in macrophage phagocytosis associated with increased mycobacterial-macrophage adhesion (2016), Front. Microbiol., 7, 1754 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.12

Cloned(Commentary)

Cloned (Comment) Organism
gene OTB2, overexpression in Mycobacterium bovis strain BCG / Pasteur 1173P2, a Mycobacterium tuberculosis variant bovis BCG, from plasmid pMV261, subcloning in Escherichia coli strain DH5alpha Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information effects of OtsB2 expression in Mycobacterium tuberculosis variant bovis BCG on mycobacterial phenotypes such as growth, phagocytosis and survival in macrophages. Mycobacterium bovis-bacillus calmette-guerin (BCG) overexpressing enzyme OtsB2 is able to better survive in stationary phase. Overexpression of enzyme OtsB2 leads to a decrease in phagocytosis but not survival in human monocyte cell-line THP-1, macrophage-like cells, and this is not due to a decrease in general macrophage phagocytic activity. Strain BCG overexpressing enzyme OtsB2 have stronger binding to THP-1 cells than wild-type BCG. Altering OtsB2 expression has implications for mycobacterial host-pathogen interactions, overview Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose 6-phosphate + H2O Mycobacterium tuberculosis
-
 alpha,alpha-trehalose + phosphate
-
 ?
alpha,alpha-trehalose 6-phosphate + H2O Mycobacterium tuberculosis H37Rv
-
 alpha,alpha-trehalose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WFZ5
-
-
Mycobacterium tuberculosis H37Rv P9WFZ5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose 6-phosphate + H2O
-
 Mycobacterium tuberculosis alpha,alpha-trehalose + phosphate
-
 ?
alpha,alpha-trehalose 6-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv alpha,alpha-trehalose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
otsB2
-
 Mycobacterium tuberculosis
TPP
-
 Mycobacterium tuberculosis
trehalose-6-phosphate phosphatase
-
 Mycobacterium tuberculosis
trehalose-phosphate phosphatase
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function trehalose-6-phosphate phosphatase (OtsB2) is involved in the OtsAB trehalose synthesis pathway to produce free trehalose and is strictly essential for mycobacterial growth Mycobacterium tuberculosis