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Literature summary for 3.1.3.12 extracted from

  • Suarez, R.; Calderon, C.; Iturriaga, G.
    Enhanced tolerance to multiple abiotic stresses in transgenic alfalfa accumulating trehalose (2009), Crop Sci., 49, 1791-1799 .
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
gene tps2, recombinant expression of a chimeric translational fusion of Saccharomyces cerevisiae trehalose-6-phosphate synthase (EC 2.4.1.15) and trehalose-6-phosphate phosphatase (EC 3.1.3.12) genes to engineer stress tolerance in alfalfa, Medicago sativa. The gene fusion TPS1-TPS2 is overexpressed in Medicago sativa cv. Regen SY27x using either the constitutive 35S promoter or the stress-regulated rd29A promoter and the Agrobacterium tumefaciens strain LBA4404-mediated transfection method Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information yeast gene encoding trehalose-6-phosphate synthase (TPS1) fused to yeast gene encoding trehalose-6-phosphate phosphatase (TPS2) gene expression under the 35S promoter in Medicago sativa plants leads to plants with stunted growth and less biomass, whereas TPS1-TPS2 expression driven by the rd29A promoter improves growth and provokes a significant increase in plant biomass at the foliage level in the different transgenic lines. Trehalose accumulates in all the different lines at similar levels under stress conditions (drought by watering withheld for 5, 10, 20, and 30 d, freezing by preadapted 5 d at 4°C before subjected to freezing stress at -5, -10, and -15°C for 6, 12, 24, 48, and 72 h, and heat-shock by treatment at 40, 45, 50, and 55°C during 1 h). Transgenic plants display a significant increase in drought, freezing, salt, and heat tolerance, phenotypes, detailed overview Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose 6-phosphate + H2O Saccharomyces cerevisiae
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 alpha,alpha-trehalose + phosphate
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 ?
alpha,alpha-trehalose 6-phosphate + H2O Saccharomyces cerevisiae ATCC 204508
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 alpha,alpha-trehalose + phosphate
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 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P31688
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Saccharomyces cerevisiae ATCC 204508 P31688
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose 6-phosphate + H2O
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 Saccharomyces cerevisiae alpha,alpha-trehalose + phosphate
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 ?
alpha,alpha-trehalose 6-phosphate + H2O
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 Saccharomyces cerevisiae ATCC 204508 alpha,alpha-trehalose + phosphate
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 ?

Synonyms

Synonyms Comment Organism
TPP
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 Saccharomyces cerevisiae
TPS2
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 Saccharomyces cerevisiae
trehalose-6-phosphate phosphatase
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 Saccharomyces cerevisiae