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Literature summary for 3.1.3.12 extracted from

  • Li, Y.T.; Zhang, H.H.; Sheng, H.M.; An, L.Z.
    Cloning, expression and characterization of trehalose-6-phosphate phosphatase from a psychrotrophic bacterium, Arthrobacter strain A3 (2012), World J. Microbiol. Biotechnol., 28, 2713-2721.
    View publication on PubMed
Search for more literature for 3.1.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 trehalose 6-phosphate at pH 7.4 and 20°C Arthrobacter sp.
0.28
-
 trehalose 6-phosphate at pH 7.4 and 10°C Arthrobacter sp.
0.39
-
 trehalose 6-phosphate at pH 7.4 and 30°C Arthrobacter sp.
0.46
-
 trehalose 6-phosphate at pH 7.4 and 4°C Arthrobacter sp.
0.51
-
 trehalose 6-phosphate at pH 7.4 and 37°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme shows an absolute requirement for Mg2+ or Co2+ for catalytic activity Arthrobacter sp.
Fe2+ in the present of Fe2+, the enzyme is less active than in the presence of Mg2+ and Co2+ Arthrobacter sp.
K+ in the present of K+, the enzyme is less active than in the presence of Mg2+ and Co2+ Arthrobacter sp.
Mg2+ the enzyme shows an absolute requirement for Mg2+ or Co2+ for catalytic activity Arthrobacter sp.
Mn2+ in the present of Mn2+, the enzyme is less active than in the presence of Mg2+ and Co2+ Arthrobacter sp.
additional information Ca2+ cannot stimulate enzyme activity and does not inhibit the effect of Mg2+ Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27873
-
 1 * 27873, calculated from amino acid sequence Arthrobacter sp.
28000
-
 1 * 28000, SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trehalose 6-phosphate + H2O Arthrobacter sp.
-
 trehalose + phosphate
-
 ?
trehalose 6-phosphate + H2O Arthrobacter sp. A3
-
 trehalose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. E9NR87
-
-
Arthrobacter sp. A3 E9NR87
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose 6-phosphate + H2O
-
 Arthrobacter sp. trehalose + phosphate
-
 ?
trehalose 6-phosphate + H2O
-
 Arthrobacter sp. A3 trehalose + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Arthrobacter sp.
monomer 1 * 27873, calculated from amino acid sequence Arthrobacter sp.

Synonyms

Synonyms Comment Organism
OtsB
-
 Arthrobacter sp.
TPP
-
 Arthrobacter sp.
trehalose-6-phosphate phosphatase
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 the activity shows a dramatic reduction at temperatures above 30°C Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 30 the enzyme exhibits full activity at 30°C, about 92% of the maximal activity at 20°C, about 85% of the maximal activity at 12°C, and still 75% of the maximal activity at 4°C Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme is generally heat-labile, losing 70% of its activity when subjected to heat treatment at 50°C for 6 min. The enzyme is completely inactivated after heat treatment at 70°C for 2 min or 60°C for 6 min Arthrobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.49
-
 trehalose 6-phosphate at pH 7.4 and 4°C Arthrobacter sp.
3.9
-
 trehalose 6-phosphate at pH 7.4 and 20°C Arthrobacter sp.
3.94
-
 trehalose 6-phosphate at pH 7.4 and 10°C Arthrobacter sp.
12.65
-
 trehalose 6-phosphate at pH 7.4 and 37°C Arthrobacter sp.
14.79
-
 trehalose 6-phosphate at pH 7.4 and 30°C Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7.5
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 the enzyme activity remains nearly constant from pH 5.0 to 7.0 in Mes/NaOH buffer and 7.0 to 7.5 in Tris/HCl buffer, whereas the enzyme shows almost no activity at pH 9.0 (Tris/HCl buffer) and half activity at pH 4.0 (acetate buffer) Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp. calculated from amino acid sequence
-
 6.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2
-
 trehalose 6-phosphate at pH 7.4 and 4°C Arthrobacter sp.
14.3
-
 trehalose 6-phosphate at pH 7.4 and 10°C Arthrobacter sp.
24.6
-
 trehalose 6-phosphate at pH 7.4 and 37°C Arthrobacter sp.
28
-
 trehalose 6-phosphate at pH 7.4 and 20°C Arthrobacter sp.
37.5
-
 trehalose 6-phosphate at pH 7.4 and 30°C Arthrobacter sp.