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Literature summary for 3.1.3.12 extracted from

  • Farelli, J.D.; Galvin, B.D.; Li, Z.; Liu, C.; Aono, M.; Garland, M.; Hallett, O.E.; Causey, T.B.; Ali-Reynolds, A.; Saltzberg, D.J.; Carlow, C.K.; Dunaway-Mariano, D.; Allen, K.N.
    Structure of the trehalose-6-phosphate phosphatase from Brugia malayi reveals key design principles for anthelmintic drugs (2014), PLoS Pathog., 10, e1004245.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli T7 Express cells Brugia malayi

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 33% (w/v) PEG 300, 0.1 M sodium citrate pH 5.0, 13% ethylene glycol and 10 mM CoCl2 Brugia malayi

Protein Variants

Protein Variants Comment Organism
D213A inactive Brugia malayi
D215A inactive Brugia malayi
D336A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
D378A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
DELTA59 the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
E384A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
E386A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
K334A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
N228A the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
Q332A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
R337A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
S329A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
T339A the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
W280A the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
Y221A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
Y225A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 6-sulfate
-
 Brugia malayi
trehalose
-
 Brugia malayi
trehalose 6-sulfate competitive inhibitor Brugia malayi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 trehalose 6-phosphate mutant enzyme N228A, at pH 7.5 and 22°C Brugia malayi
0.046
-
 trehalose 6-phosphate mutant enzyme D336A, at pH 7.5 and 22°C Brugia malayi
0.082
-
 trehalose 6-phosphate mutant enzyme E386A, at pH 7.5 and 22°C Brugia malayi
0.1
-
 trehalose 6-phosphate mutant enzyme E384A, at pH 7.5 and 22°C Brugia malayi
0.15
-
 trehalose 6-phosphate mutant enzyme W280A, at pH 7.5 and 22°C Brugia malayi
0.15
-
 trehalose 6-phosphate mutant enzyme Y225A, at pH 7.5 and 22°C Brugia malayi
0.2
-
 trehalose 6-phosphate mutant enzyme Q332A, at pH 7.5 and 22°C Brugia malayi
0.2
-
 trehalose 6-phosphate mutant enzyme T339A, at pH 7.5 and 22°C Brugia malayi
0.25
-
 trehalose 6-phosphate mutant enzyme S329A, at pH 7.5 and 22°C Brugia malayi
0.26
-
 trehalose 6-phosphate mutant enzyme DELTA59, at pH 7.5 and 22°C Brugia malayi
0.27
-
 trehalose 6-phosphate mutant enzyme D378A, at pH 7.5 and 22°C Brugia malayi
0.33
-
 trehalose 6-phosphate mutant enzyme Y221A, at pH 7.5 and 22°C Brugia malayi
0.36
-
 trehalose 6-phosphate wild type enzyme, at pH 7.5 and 22°C Brugia malayi
0.4
-
 trehalose 6-phosphate mutant enzyme R337A, at pH 7.5 and 22°C Brugia malayi
0.5
-
 trehalose 6-phosphate mutant enzyme K334A, at pH 7.5 and 22°C Brugia malayi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Brugia malayi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trehalose 6-phosphate + H2O Brugia malayi
-
 trehalose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Brugia malayi A8NS89
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography Brugia malayi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose 6-phosphate + H2O
-
 Brugia malayi trehalose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GOB-1
-
 Brugia malayi
T6PP
-
 Brugia malayi
trehalose 6-phosphate phosphatase
-
 Brugia malayi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 trehalose 6-phosphate mutant enzyme K334A, at pH 7.5 and 22°C Brugia malayi
0.094
-
 trehalose 6-phosphate mutant enzyme D336A, at pH 7.5 and 22°C Brugia malayi
0.22
-
 trehalose 6-phosphate mutant enzyme Q332A, at pH 7.5 and 22°C Brugia malayi
0.28
-
 trehalose 6-phosphate mutant enzyme R337A, at pH 7.5 and 22°C Brugia malayi
1
-
 trehalose 6-phosphate mutant enzyme Y221A, at pH 7.5 and 22°C Brugia malayi
2 8 trehalose 6-phosphate mutant enzyme DELTA59, at pH 7.5 and 22°C Brugia malayi
2
-
 trehalose 6-phosphate mutant enzyme E386A, at pH 7.5 and 22°C Brugia malayi
3
-
 trehalose 6-phosphate mutant enzyme E384A, at pH 7.5 and 22°C Brugia malayi
4
-
 trehalose 6-phosphate mutant enzyme S329A, at pH 7.5 and 22°C Brugia malayi
6
-
 trehalose 6-phosphate mutant enzyme Y225A, at pH 7.5 and 22°C Brugia malayi
11
-
 trehalose 6-phosphate mutant enzyme D378A, at pH 7.5 and 22°C Brugia malayi
15
-
 trehalose 6-phosphate mutant enzyme T339A, at pH 7.5 and 22°C Brugia malayi
18
-
 trehalose 6-phosphate mutant enzyme N228A, at pH 7.5 and 22°C Brugia malayi
19
-
 trehalose 6-phosphate mutant enzyme W280A, at pH 7.5 and 22°C Brugia malayi
24
-
 trehalose 6-phosphate wild type enzyme, at pH 7.5 and 22°C Brugia malayi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.082
-
 trehalose 6-sulfate wild type enzyme, at pH 7.5 and 22°C Brugia malayi
10
-
 trehalose Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C Brugia malayi
10
-
 D-glucose 6-sulfate Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C Brugia malayi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.11
-
 trehalose 6-phosphate mutant enzyme K334A, at pH 7.5 and 22°C Brugia malayi
0.2
-
 trehalose 6-phosphate mutant enzyme D336A, at pH 7.5 and 22°C Brugia malayi
0.55
-
 trehalose 6-phosphate mutant enzyme Q332A, at pH 7.5 and 22°C Brugia malayi
1.5
-
 trehalose 6-phosphate mutant enzyme R337A, at pH 7.5 and 22°C Brugia malayi
3
-
 trehalose 6-phosphate mutant enzyme Y221A, at pH 7.5 and 22°C Brugia malayi
15
-
 trehalose 6-phosphate mutant enzyme S329A, at pH 7.5 and 22°C Brugia malayi
28
-
 trehalose 6-phosphate mutant enzyme E386A, at pH 7.5 and 22°C Brugia malayi
30
-
 trehalose 6-phosphate mutant enzyme E384A, at pH 7.5 and 22°C Brugia malayi
36
-
 trehalose 6-phosphate mutant enzyme Y225A, at pH 7.5 and 22°C Brugia malayi
39
-
 trehalose 6-phosphate mutant enzyme D378A, at pH 7.5 and 22°C Brugia malayi
69
-
 trehalose 6-phosphate wild type enzyme, at pH 7.5 and 22°C Brugia malayi
77
-
 trehalose 6-phosphate mutant enzyme T339A, at pH 7.5 and 22°C Brugia malayi
110
-
 trehalose 6-phosphate mutant enzyme DELTA59, at pH 7.5 and 22°C Brugia malayi
120
-
 trehalose 6-phosphate mutant enzyme W280A, at pH 7.5 and 22°C Brugia malayi
450
-
 trehalose 6-phosphate mutant enzyme N228A, at pH 7.5 and 22°C Brugia malayi