Literature summary for 3.1.3.12 extracted from

Lahiri, S.; Banerjee, S.; Dutta, T.; Sengupta, S.; Dey, S.; Roy, R.; Sengupta, D.; Chattopadhyay, K.; Ghosh, A.K.
Enzymatic and regulatory attributes of trehalose-6-phosphate phosphatase from Candida utilis and its role during thermal stress (2014), J. Cell. Physiol., 229, 1245-1255.

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Inhibitors

Inhibitors	Comment	Organism	Structure
CDTA	strong inhibition	Cyberlindnera jadinii
EDTA	strong inhibition	Cyberlindnera jadinii
EGTA	strong inhibition	Cyberlindnera jadinii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.82	-	trehalose 6-phosphate	native enzyme, at pH 8.5 and 30°C	Cyberlindnera jadinii
0.9	-	trehalose 6-phosphate	recombinant enzyme, at pH 8.5 and 30°C	Cyberlindnera jadinii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	has significant positive effect on enzyme activity	Cyberlindnera jadinii
Mg2+	required, almost 10fold increase in activity at 2 mM	Cyberlindnera jadinii
Mn2+	has significant positive effect on enzyme activity	Cyberlindnera jadinii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
82000	-	x * 82000, SDS-PAGE	Cyberlindnera jadinii
82108	-	x * 82108, MALDI-TOF mass spectrometry	Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trehalose 6-phosphate + H2O	Cyberlindnera jadinii	-	trehalose + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Cyberlindnera jadinii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Protein-Pak DEAE column chromatography and Biosuite 125 gel filtration	Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.01033	-	cell lysate, at pH 8.5 and 30°C	Cyberlindnera jadinii
0.4505	-	after 43.6fold purification, at pH 8.5 and 30°C	Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	no activity with sucrose-6-phosphate, fructose-6-phosphate, glucose-6-phosphate, fructose-6-phosphate, mannose-6-phosphate, 4-nitrophenylphosphate, ATP, and ADP	Cyberlindnera jadinii	?	-	?
trehalose 6-phosphate + H2O	-	Cyberlindnera jadinii	trehalose + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 82000, SDS-PAGE	Cyberlindnera jadinii
?	x * 82108, MALDI-TOF mass spectrometry	Cyberlindnera jadinii

Synonyms

Synonyms	Comment	Organism
TPP	-	Cyberlindnera jadinii
trehalose-6-phosphate phosphatase	-	Cyberlindnera jadinii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Cyberlindnera jadinii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	60	the enzyme is most stable up to 30°C. The enzyme is also able to show almost 50% activity at temperatures as high as 60°C	Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Cyberlindnera jadinii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	12	the enzyme retains almost 60% activity in highly alkaline condition (pH 10.0) or moderately acidic condition (pH 6.0). Almost 40% activity still remains in pH as low as 4.0 or as high as 12.0	Cyberlindnera jadinii

Expression

Organism	Comment	Expression
Cyberlindnera jadinii	almost 1.8fold increase in activity is observed when the concentration of S-adenosyl-L-methionine is increased from 0 to 2 mM	up

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Release 2023.1 (January 2023)