Any feedback?
Literature summary for 3.1.3.12 extracted from
- A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT (2008), J. Bacteriol., 190, 7939-7946.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Rubrobacter xylanophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.1 | - |
trehalose 6-phosphate | Vmax: 225 microgram/min/mg protein | Rubrobacter xylanophilus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2mM Mg2+ or Co2+ | Rubrobacter xylanophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29400 | - |
deduced from cDNA | Rubrobacter xylanophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rubrobacter xylanophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Rubrobacter xylanophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + H2O | - |
Rubrobacter xylanophilus | trehalose + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPP | - |
Rubrobacter xylanophilus |
| trehalose 6-phosphate phosphatase | - |
Rubrobacter xylanophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at and optimal activity | Rubrobacter xylanophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 3.2 h | Rubrobacter xylanophilus |
| 70 | - |
half-life: 1.0 h | Rubrobacter xylanophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rubrobacter xylanophilus |
| 7.5 | - |
optimal activity | Rubrobacter xylanophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
