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Literature summary for 3.1.3.12 extracted from

  • Rao, K.N.; Kumaran, D.; Seetharaman, J.; Bonanno, J.B.; Burley, S.K.; Swaminathan, S.
    Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications (2006), Protein Sci., 15, 1735-1744.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.12

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 30% PEG 4000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5) Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 trehalose 6-phosphate in 50 mM Tris-HCl (pH 8.0), 5 mM dithiothreitol, and 20 mM MgCl2, at 50°C Thermoplasma acidophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-
Thermoplasma acidophilum TA1209
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and S200 gel filtration Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose 6-phosphate + H2O
-
 Thermoplasma acidophilum trehalose + phosphate
-
 ?
trehalose 6-phosphate + H2O
-
 Thermoplasma acidophilum TA1209 trehalose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
T6PP
-
 Thermoplasma acidophilum
trehalose-6-phosphate phosphatase-related protein
-
 Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
 trehalose 6-phosphate in 50 mM Tris-HCl (pH 8.0), 5 mM dithiothreitol, and 20 mM MgCl2, at 50°C Thermoplasma acidophilum