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Literature summary for 3.1.3.12 extracted from

  • Shima, S.; Matsui, H.; Tahara, S.; Imai, R.
    Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes (2007), FEBS J., 274, 1192-1201.
    View publication on PubMed
Search for more literature for 3.1.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Oryza sativa Japonica Group
expressed in Escherichia coli BL21 cells Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0921
-
 trehalose 6-phosphate in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C Oryza sativa
0.186
-
 trehalose 6-phosphate in 50 mM Tris/HCl buffer with 2 mM MgCl2, at 37°C Oryza sativa Japonica Group

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42600
-
 deduced from the nucleotide sequence Oryza sativa Japonica Group
45000
-
 SDS-PAGE Oryza sativa Japonica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Q75WV3 cultivar Yukihikari
-
Oryza sativa Japonica Group Q9FWQ2 cultivar Yukihikari
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B affinity column chromatography Oryza sativa Japonica Group
glutathione-Sepharose 4B affinity column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Oryza sativa Japonica Group
-
root
-
 Oryza sativa
-
shoot
-
 Oryza sativa Japonica Group
-
shoot
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information almost no activity with glucose 1-phosphate, glucose 6-phosphate, galactose 6-phosphate, mannose 1-phosphate, mannose 6-phosphate, fructose 1-phosphate, fructose 6-phosphate, sucrose 6-phosphate, lactose 1-phosphate, and ribose 5-phosphate Oryza sativa Japonica Group ?
-
 ?
additional information almost no activity with glucose 1-phosphate, glucose 6-phosphate, galactose 6-phosphate, mannose 1-phosphate, mannose 6-phosphate, fructose 1-phosphate, fructose 6-phosphate, sucrose 6-phosphate, lactose 1-phosphate, and ribose 5-phosphate Oryza sativa ?
-
 ?
trehalose 6-phosphate + H2O
-
 Oryza sativa Japonica Group trehalose + phosphate
-
 ?
trehalose 6-phosphate + H2O
-
 Oryza sativa trehalose + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Tpp1
-
 Oryza sativa
TPP2
-
 Oryza sativa Japonica Group
trehalose-6-phosphate phosphatase
-
 Oryza sativa Japonica Group
trehalose-6-phosphate phosphatase
-
 Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 almost completely loses activity when subjected to heat treatment at 50°C for 4 min Oryza sativa Japonica Group
50
-
 almost completely loses activity when subjected to heat treatment at 50°C for 4 min Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 trehalose 6-phosphate in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C Oryza sativa
6.52
-
 trehalose 6-phosphate in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C Oryza sativa
13.4
-
 trehalose 6-phosphate in 50 mM Tris/HCl buffer with 2 mM MgCl2, at 37°C Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Oryza sativa Japonica Group
7
-
-
 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Oryza sativa Japonica Group
5.5 9
-
 Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 almost no activity at pH 5.5 or 9.0 Oryza sativa Japonica Group
5.5 9 almost no activity at pH 5.5 or 9.0 Oryza sativa