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Literature summary for 3.1.3.12 extracted from
- Occurence of trehalose-6-phosphatase in Phormia regina Meig. (1960), Arch. Biochem. Biophys., 88, 339-343.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenate | 10 mM, 10% inhibition | Phormia regina |  |
| Cu2+ | 0.6 mM, 50% inhibition in presence of 5 mM Mg2+ | Phormia regina | |
| F- | 25 mM, 70% inhibition | Phormia regina | |
| Zn2+ | 0.6 mM, 80% inhibition in presence of 5 mM Mg2+ | Phormia regina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately | Phormia regina | |
| Fe2+ | enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately | Phormia regina | |
| Mg2+ | enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately | Phormia regina | |
| Mn2+ | enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately | Phormia regina | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phormia regina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phormia regina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Phormia regina |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -12°C, completely stable for at least 2 months | Phormia regina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glucose 6-phosphate + H2O | 7% of the activity with trehalose 6-phosphate | Phormia regina | ? | - |
? | |
| trehalose-6-phosphate + H2O | - |
Phormia regina | trehalose + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | - |
- |
Phormia regina |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 58 | 30°C: about 45% of maximal activity, 58°C: about 85% of mmaximal activity | Phormia regina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
15 min, 15% loss of activity | Phormia regina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Phormia regina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.2 | pH 5.8: about 20% of maximal activity, pH 8.2: about 75% of maximal activity | Phormia regina |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Cu2+ | in presence of 5 mM Mg2+ | Phormia regina | |
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