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Literature summary for 3.1.3.12 extracted from

  • Friedman, S.
    Trehalose-6-phosphate phosphatase from insects (1966), Methods Enzymol., 8, 372-374.
No PubMed abstract available
Search for more literature for 3.1.3.12

Inhibitors

Inhibitors Comment Organism Structure
arsenate 10 mM, 10% inhibition Phormia regina
Cu2+ 0.6 mM, 50% inhibition in presence of 5 mM Mg2+ Phormia regina
F- 25 mM, 70% inhibition Phormia regina
Zn2+ 0.6 mM, 80% inhibition in presence of 5 mM Mg2+ Phormia regina

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina
Fe2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina
Mg2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina
Mn2+ enzyme is inactive in absence of a bivalent metal. Requirement is met most effectively by Mg2+ with Co2+, Fe2+ and Mn2+ serving much less adequately Phormia regina

Organism

Organism UniProt Comment Textmining
Phormia regina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phormia regina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.61
-
-
 Phormia regina

Storage Stability

Storage Stability Organism
-12°C, completely stable for at least 2 months Phormia regina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose-6-phosphate + H2O
-
 Phormia regina trehalose + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
46
-
-
 Phormia regina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 15 min, 15% loss of activity Phormia regina

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 Cu2+ in presence of 5 mM Mg2+ Phormia regina