Any feedback?
Literature summary for 3.1.3.11 extracted from
- A new level of regulation in gluconeogenesis metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase (2015), Biochem. J., 472, 225-237 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of GST-tagged liver enzyme in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Rattus norvegicus |  |
| fructose-1,6-bisphosphate | at higher concentrations | Rattus norvegicus | |
| fructose-2,6-bisphosphate | - |
Rattus norvegicus | |
| fructose-6-phosphate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | in absence of glucose | Rattus norvegicus | 5737 | - |
| nucleus | when the cells are incubated with 25 mM glucose, the enzyme changes its cellular localization, FBPase-1 becomes more concentrated in the nucleus, but the enzyme still shows an important co-localization in the cellular periphery. In presence of 25 mM glucose and 100 nM insulin, FBPase-1 mainly translocates from cytoplasm to the nucleus | Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Rattus norvegicus | |
| Mg2+ | activates | Rattus norvegicus | |
| Mn2+ | activates | Rattus norvegicus | |
| Zn2+ | activates | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Rattus norvegicus |
| 36000 | - |
gel filtration, recombinant enzyme | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Rattus norvegicus | - |
D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | Rattus norvegicus Wistar | - |
D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P19112 | - |
- |
| Rattus norvegicus Wistar | P19112 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| recombinant GST-tagged liver enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatocyte | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| additional information | coexpression of aldolase B and FBPase-1 in cultured cells | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Rattus norvegicus | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Rattus norvegicus Wistar | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FBPase-1 | - |
Rattus norvegicus |
| fructose-1,6-bisphosphatase | - |
Rattus norvegicus |
| fructose-1,6-bisphosphatase-1 | - |
Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| drug target | the enzyme (FBPase-1) is considered to be a new target for the control of diabetes | Rattus norvegicus |
| metabolism | metabolic conditions modulate aldolase B and FBPase-1 activity at the cellular level through the regulation of their interaction, suggesting that their association confers a catalytic advantage for both enzymes | Rattus norvegicus |
| physiological function | fructose-1,6-bisphosphatase is a highly regulated key enzyme in gluconeogenesis and glyconeogenesis (glycogen synthesis from gluconeogenic precursors). The enzyme catalyses the hydrolysis of fructose-1,6-bisphosphate to fructose 6-phosphate and phosphate in the presence of divalent metal cations. In vitro protein-protein interaction analysis between liver fructose 1,6-bisphosphate aldolase B and liver FBPase-1 shows a specific and regulable interaction between them, whereas aldolase A (muscle isozyme) and FBPase-1 show no interaction, by real-time interaction analysis by surface plasmon resonance. The interaction between aldolase B and FBPase-1 is specific and reversible. The affinity of the aldolase B and FBPase-1 complex is modulated by intermediate metabolites, but only in the presence of K+. A decreased association constant is observed in the presence of adenosine monophosphate, fructose-2,6-bisphosphate, fructose-6-phosphate and inhibitory concentrations of fructose-1,6-bisphosphate. Conversely, the association constant of the complex increases in the presence of dihydroxyacetone phosphate (DHAP) and non-inhibitory concentrations of fructose-1,6-bisphosphate | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
