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Literature summary for 3.1.3.11 extracted from
- Characterization of fructose 1,6-bisphosphatase and sedoheptulose 1,7-bisphosphatase from the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus (2013), J. Bacteriol., 195, 5112-5122.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the substrate for the sedoheptulose 1,7-bisphosphatase reaction, sedoheptulose 1,7-bisphosphate, can be synthesized in vitro by using both the chromosomal-encoded or the plasmid-encoded fructose 1,6-bisphosphate aldolase proteins from Bacillus methanolicus | Bacillus methanolicus |
| synthesis | the substrate for the sedoheptulose 1,7-bisphosphatase reaction, sedoheptulose 1,7-bisphosphate, can be synthesized in vitro by using both the chromosomal-encoded or theplasmid-encoded fructose 1,6-bisphosphate aldolase proteins from Bacillus methanolicus | Bacillus methanolicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli, Bacillus methanolicus MGA3 | Bacillus methanolicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 33% inhibition; 38% inhibition | Bacillus methanolicus |  |
| ATP | 50% inhibition; 56% inhibition | Bacillus methanolicus | |
| D-fructose 1,6-bisphosphate | - |
Bacillus methanolicus | |
| EDTA | at an equimolar concentration to the bivalent metal ions, almost complete loss of activity; at an equimolar concentration to the bivalent metal ions, almost complete loss of activity | Bacillus methanolicus | |
| Li+ | 1 mM, 50% residual activity; 1 mM, 50% residual activity | Bacillus methanolicus | |
| additional information | not inhibitory: AMP, phosphoenolpyruvate, D-fructose 1-phosphate, D-fructose 6-phosphate, and D-fructose 2,6-bisphosphate up to 5 mM; not inhibitory: AMP, phosphoenolpyruvate, D-fructose 1-phosphate, D-fructose 6-phosphate, and D-fructose 2,6-bisphosphate up to 5 mM | Bacillus methanolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50° | Bacillus methanolicus | |
| 0.44 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50° | Bacillus methanolicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 100 mM, 20% increase in activity | Bacillus methanolicus | |
| Mn2+ | activation | Bacillus methanolicus | |
| additional information | no significant activation: Co2+, Ni2+, Cu2+, Zn2+, Fe2+, and Ca2+ | Bacillus methanolicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33132 | - |
4 * 33132, calculated | Bacillus methanolicus |
| 35145 | - |
4 * 35145, calculated | Bacillus methanolicus |
| 135000 | - |
gel filtration | Bacillus methanolicus |
| 142000 | - |
gel filtration | Bacillus methanolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus methanolicus | I3DTM3 | chromosome-encoded isoform; i.e. MGA3 | - |
| Bacillus methanolicus | Q6TV42 | plasmid-encoded isoform | - |
| Bacillus methanolicus ATCC 53907 | I3DTM3 | chromosome-encoded isoform; i.e. MGA3 | - |
| Bacillus methanolicus ATCC 53907 | Q6TV42 | plasmid-encoded isoform | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Bacillus methanolicus | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Bacillus methanolicus ATCC 53907 | D-fructose 6-phosphate + phosphate | - |
? | |
| additional information | the chromosome-encoded isoform is the major fructose 1,6-bisphosphatase of Bacillus methanolicus | Bacillus methanolicus | ? | - |
? | |
| additional information | the chromosome-encoded isoform is the major fructose 1,6-bisphosphatase of Bacillus methanolicus | Bacillus methanolicus ATCC 53907 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33132, calculated | Bacillus methanolicus |
| tetramer | 4 * 35145, calculated | Bacillus methanolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlpXp | - |
Bacillus methanolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Bacillus methanolicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50° | Bacillus methanolicus | |
| 3.9 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50° | Bacillus methanolicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Bacillus methanolicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Bacillus methanolicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50°C | Bacillus methanolicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | a fructose bisphosphatase-negative Corynebacterium glutamicum mutant can be phenotypically complemented with both the chromosome-encoded and the plasmid-encoded isoforms from Bacillus methanolicus | Bacillus methanolicus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50° | Bacillus methanolicus | |
| 86.3 | - |
D-fructose 1,6-bisphosphate | pH 7.7, 50° | Bacillus methanolicus | |
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