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Literature summary for 3.1.3.11 extracted from
- Thermus thermophilus encodes an archaeal-like fructose-1,6-bisphosphatase: purification of native and recombinant protein for structural studies (2010), Protein Expr. Purif., 74, 175-180.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS | Thermus thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 20°C, X-ray diffraction structure determination and analysis of twinned crystals at 1.2 A resolution, and of untwinned crystals at 1.8 A resolution | Thermus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40324 | - |
2 * 40324, mass spectrometry, 2 * 40361, sequence calculation | Thermus thermophilus |
| 40361 | - |
2 * 40324, mass spectrometry, 2 * 40361, sequence calculation | Thermus thermophilus |
| 80000 | - |
gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Thermus thermophilus | - |
D-fructose 6-phosphate + phosphate | - |
? |  |
| D-fructose 1,6-bisphosphate + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q5SJM8 | ATCC 27634 | - |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJM8 | ATCC 27634 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by heat treatment, nickel affinity chromatography, and gel filtration | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Thermus thermophilus | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40324, mass spectrometry, 2 * 40361, sequence calculation | Thermus thermophilus |
| More | in solution, the enzyme appears to be a homodimer which ensembles into an octamer in the tetragonal crystals | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| archaeal-like fructose-1,6-bisphosphatase | - |
Thermus thermophilus |
| FBPase | - |
Thermus thermophilus |
| fructose-1,6-bisphosphatase | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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