Cloned (Comment) | Organism |
---|---|
subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3) | Thermococcus onnurineus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Thermococcus onnurineus | |
ATP | - |
Thermococcus onnurineus | |
Ca2+ | - |
Thermococcus onnurineus | |
additional information | no inhibition by AMP | Thermococcus onnurineus | |
phosphoenolpyruvate | - |
Thermococcus onnurineus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates 5fold at 1 mM | Thermococcus onnurineus | |
additional information | no activation by Li+ | Thermococcus onnurineus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Thermococcus onnurineus | - |
D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus onnurineus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Thermococcus onnurineus | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | best substrate | Thermococcus onnurineus | D-fructose 6-phosphate + phosphate | - |
? | |
additional information | the enzyme TNA1-Fbp has broad substrate specificities for D-fructose-1,6-bisphosphate and its analogues including D-fructose 1-bisphosphate, D-fructose 6-phosphate, D-glucose 1-phosphate, D-glucose 6-phosphate, glycerol 2-phosphate, and phosphoenolpyruvate, as well as AMP, ADP ATP, substrate specificity, overview | Thermococcus onnurineus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class V FBPase | - |
Thermococcus onnurineus |
class V fructose-1,6-bisphosphatase | - |
Thermococcus onnurineus |
FBPase | - |
Thermococcus onnurineus |
fructose-1,6-bisphosphatase | - |
Thermococcus onnurineus |
TNA1-Fbp | - |
Thermococcus onnurineus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Thermococcus onnurineus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
purified recombinant enzyme, pH 8.0, half-life is 8 h | Thermococcus onnurineus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermococcus onnurineus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
purified recombinant enzyme, 95°C, half-life is 8 h | Thermococcus onnurineus |