Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | acts synergistically with fructose-2,6-bisphosphate on the frog muscle FBPase | Pelophylax lessonae | |
Ca2+ | - |
Pelophylax lessonae | |
D-fructose 1,6-bisphosphate | substrate inhibition at higher concentrations | Pelophylax lessonae | |
D-fructose-2,6-bisphosphate | acts synergistically with AMP on the frog muscle FBPase | Pelophylax lessonae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Pelophylax lessonae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Pelophylax lessonae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Pelophylax lessonae | - |
D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelophylax lessonae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by heat treatment at 61°C for 2 min, ammonium sulfate fractionation, dialysis and adosption chromatography on a phosphocellulose resin with elution by inhibitors AMP and fructose-2,6-bisphosphate | Pelophylax lessonae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Pelophylax lessonae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
purified enzyme, pH 7.5, 25°C | Pelophylax lessonae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Pelophylax lessonae | D-fructose 6-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Pelophylax lessonae |
Synonyms | Comment | Organism |
---|---|---|
FBPase | - |
Pelophylax lessonae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pelophylax lessonae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pelophylax lessonae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | activity range | Pelophylax lessonae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000028 | - |
D-fructose-2,6-bisphosphate | pH 7.5, 25°C, in presence of 500 nM AMP | Pelophylax lessonae | |
0.000092 | - |
D-fructose-2,6-bisphosphate | pH 7.5, 25°C, in presence of 50 nM AMP | Pelophylax lessonae | |
0.000114 | - |
D-fructose-2,6-bisphosphate | pH 7.5, 25°C | Pelophylax lessonae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0002 | - |
pH 7.5, 25°C | Pelophylax lessonae | AMP | |
0.055 | - |
pH 7.5, 25°C | Pelophylax lessonae | D-fructose 1,6-bisphosphate | |
0.091 | - |
pH 7.5, 25°C | Pelophylax lessonae | Ca2+ |