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Literature summary for 3.1.3.11 extracted from

  • Dziewulska-Szwajkowska, D.; Dzugaj, A.
    Kinetic properties of Pelophylax esculentus muscle FBPase (2010), Comp. Biochem. Physiol. B, 157, 294-300.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AMP acts synergistically with fructose-2,6-bisphosphate on the frog muscle FBPase Pelophylax lessonae
Ca2+
-
Pelophylax lessonae
D-fructose 1,6-bisphosphate substrate inhibition at higher concentrations Pelophylax lessonae
D-fructose-2,6-bisphosphate acts synergistically with AMP on the frog muscle FBPase Pelophylax lessonae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Pelophylax lessonae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Pelophylax lessonae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Pelophylax lessonae
-
D-fructose 6-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pelophylax lessonae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by heat treatment at 61°C for 2 min, ammonium sulfate fractionation, dialysis and adosption chromatography on a phosphocellulose resin with elution by inhibitors AMP and fructose-2,6-bisphosphate Pelophylax lessonae

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Pelophylax lessonae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
purified enzyme, pH 7.5, 25°C Pelophylax lessonae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Pelophylax lessonae D-fructose 6-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Pelophylax lessonae

Synonyms

Synonyms Comment Organism
FBPase
-
Pelophylax lessonae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pelophylax lessonae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pelophylax lessonae

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 activity range Pelophylax lessonae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000028
-
D-fructose-2,6-bisphosphate pH 7.5, 25°C, in presence of 500 nM AMP Pelophylax lessonae
0.000092
-
D-fructose-2,6-bisphosphate pH 7.5, 25°C, in presence of 50 nM AMP Pelophylax lessonae
0.000114
-
D-fructose-2,6-bisphosphate pH 7.5, 25°C Pelophylax lessonae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0002
-
pH 7.5, 25°C Pelophylax lessonae AMP
0.055
-
pH 7.5, 25°C Pelophylax lessonae D-fructose 1,6-bisphosphate
0.091
-
pH 7.5, 25°C Pelophylax lessonae Ca2+