Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging-drop vapor-diffusion method, 0.001 ml of protein solution containing 10 mg/ml protein, is mixed with 0.001 ml of reservoir solution containing 1.8 M ammonium citrate tribasic, pH 7.0, and equilibratzion against 0.3 ml reservoir solution, 72 h to 2 weeks, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Mycobacterium tuberculosis | - |
D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Mycobacterium tuberculosis H37Rv | - |
D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene glpX | - |
Mycobacterium tuberculosis H37Rv | - |
gene glpX | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Mycobacterium tuberculosis | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | D-fructose 6-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
class II FBPase | - |
Mycobacterium tuberculosis |
class II fructose-1,6-bisphosphatase | - |
Mycobacterium tuberculosis |
FBPase | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis | Mycobacterium tuberculosis |