Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Pisum sativum |
Protein Variants | Comment | Organism |
---|---|---|
K115Q | Km (D-fructose 1,6-bisphosphate) and Ki (D-fructose 2,6-bisphosphate) comparable to wild-type, IC50 (AMP): 5.8 mM | Pisum sativum |
Y116Q | Km (D-fructose 1,6-bisphosphate) and Ki (D-fructose 2,6-bisphosphate) comparable to wild-type, IC50 (AMP): 15.9 mM | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Pisum sativum | |
D-fructose 1,6-bisphosphate | - |
Pisum sativum | |
D-fructose-2,6-bisphosphate | - |
Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
D-fructose 1,6-bisphosphate | mutant Y116F | Pisum sativum | |
0.0107 | - |
D-fructose 1,6-bisphosphate | mutant K115Q | Pisum sativum | |
0.0108 | - |
D-fructose 1,6-bisphosphate | wild-type | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pisum sativum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Pisum sativum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.1 | - |
wild-type protein, after Ni-NTA chromatography | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Pisum sativum | D-fructose 6-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
D-fructose 1,6-bisphosphatase | - |
Pisum sativum |
FBPase | - |
Pisum sativum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
D-fructose 1,6-bisphosphate | mutant K115Q | Pisum sativum | |
0.014 | - |
D-fructose 1,6-bisphosphate | mutant Y116F | Pisum sativum | |
0.015 | - |
D-fructose 2,6-bisphosphate | wild-type | Pisum sativum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
wild-type | Pisum sativum | AMP | |
5.81 | - |
mutant K115Q | Pisum sativum | AMP | |
15.96 | - |
mutant Y116F | Pisum sativum | AMP |