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Literature summary for 3.1.3.11 extracted from

  • Rashid, N.; Imanaka, H.; Kanai, T.; Fukui, T.; Atomi, H.; Imanaka, T.
    A novel candidate for the true fructose-1,6-bisphosphatase in Archaea (2002), J. Biol. Chem., 277, 30649-30655.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol in combination wit Mg2+ Thermococcus kodakarensis

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
D-fructose-2,6-bisphosphate
-
Thermococcus kodakarensis
additional information not: AMP Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
D-fructose 1,6-bisphosphate 95°C Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Thermococcus kodakarensis
Mn2+ 1 mM, 4fold increase of activity Thermococcus kodakarensis
additional information not: Ca2+, Ni2+ Thermococcus kodakarensis
Zn2+ 1 mM Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
8 * 42000, SDS-PAGE Thermococcus kodakarensis
340000
-
gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Thermococcus kodakarensis enzyme is usually regarded as a regulatory enzyme of gluconeogenesis D-fructose 6-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q8NKR9 KOD1
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant enzyme Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Thermococcus kodakarensis D-fructose 6-phosphate + phosphate
-
?
D-fructose 1,6-bisphosphate + H2O enzyme is usually regarded as a regulatory enzyme of gluconeogenesis Thermococcus kodakarensis D-fructose 6-phosphate + phosphate
-
?
additional information not: D-fructose 1-phosphate, D-fructose 6-phosphate, D-fructose 2,6-bisphosphate, glycerol 3-phosphate, glycerol 1-phosphate Thermococcus kodakarensis ?
-
?

Subunits

Subunits Comment Organism
octamer 8 * 42000, SDS-PAGE Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
in presence of 20 mM Mg2+ and 20 mM dithioerythritol Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 95 the enzyme shows a nearly linear increase in activity between 37°C and 95°C Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
half-life of 150 min in boiling water Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
D-fructose 1,6-bisphosphate 95°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
in presence of 20 mM Mg2+ and 20 mM dithioerythritol Thermococcus kodakarensis