Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | in combination wit Mg2+ | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose-2,6-bisphosphate | - |
Thermococcus kodakarensis | |
additional information | not: AMP | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-fructose 1,6-bisphosphate | 95°C | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Thermococcus kodakarensis | |
Mn2+ | 1 mM, 4fold increase of activity | Thermococcus kodakarensis | |
additional information | not: Ca2+, Ni2+ | Thermococcus kodakarensis | |
Zn2+ | 1 mM | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
8 * 42000, SDS-PAGE | Thermococcus kodakarensis |
340000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Thermococcus kodakarensis | enzyme is usually regarded as a regulatory enzyme of gluconeogenesis | D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q8NKR9 | KOD1 | - |
Purification (Comment) | Organism |
---|---|
the recombinant enzyme | Thermococcus kodakarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Thermococcus kodakarensis | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | enzyme is usually regarded as a regulatory enzyme of gluconeogenesis | Thermococcus kodakarensis | D-fructose 6-phosphate + phosphate | - |
? | |
additional information | not: D-fructose 1-phosphate, D-fructose 6-phosphate, D-fructose 2,6-bisphosphate, glycerol 3-phosphate, glycerol 1-phosphate | Thermococcus kodakarensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 42000, SDS-PAGE | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
in presence of 20 mM Mg2+ and 20 mM dithioerythritol | Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 95 | the enzyme shows a nearly linear increase in activity between 37°C and 95°C | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life of 150 min in boiling water | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
D-fructose 1,6-bisphosphate | 95°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in presence of 20 mM Mg2+ and 20 mM dithioerythritol | Thermococcus kodakarensis |