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Literature summary for 3.1.3.11 extracted from

  • Reichert, A.; Dennes, A.; Vetter, S.; Scheibe, R.
    Chloroplast fructose 1,6-bisphosphatase with changed redox modulation: comparison of the Galdieria enzyme with cysteine mutants from spinach (2003), Biochim. Biophys. Acta, 1645, 212-217.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Spinacia oleracea
expression in Escherichia coli, in this sequence of enzyme, the Cys that corresponds to Cys179 in the spinach enzyme is lacking Galdieria sulphuraria

Protein Variants

Protein Variants Comment Organism
C155S mutant is active Spinacia oleracea
C174S mutant is active and can be inactivated by oxidation Spinacia oleracea
C179S mutant is inactive Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, comparison of activities of wild-type and mutant enzymes in dependence of Mg2+ concentrations Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Galdieria sulphuraria enzyme is a part of Calvin cycle D-fructose 6-phosphate + phosphate
-
?
D-fructose 1,6-diphosphate + H2O Spinacia oleracea enzyme is a part of Calvin cycle D-fructose 6-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Brassica napus
-
-
-
Galdieria sulphuraria Q95AJ2 red alga
-
Glycine max
-
-
-
Oryza sativa
-
rice
-
Pisum sativum
-
pea
-
Solanum tuberosum
-
potato
-
Spinacia oleracea
-
-
-
Triticum aestivum
-
wheat
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea
-
Galdieria sulphuraria

Reaction

Reaction Comment Organism Reaction ID
D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate the chloroplast enzyme of higher plants can occur in an active form that is inactivated upon disulfide bridge formation between Cys155 and Cys174 Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
comparison of activities of oxidizied and reduced enzyme Galdieria sulphuraria
additional information
-
comparison of activities of oxidizied and reduced wild-type and mutant enzymes Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
Galdieria sulphuraria D-fructose 6-phosphate + phosphate
-
?
D-fructose 1,6-bisphosphate + H2O enzyme is a part of Calvin cycle Galdieria sulphuraria D-fructose 6-phosphate + phosphate
-
?
D-fructose 1,6-diphosphate + H2O enzyme is a part of Calvin cycle Spinacia oleracea D-fructose 6-phosphate + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
under reducing conditions Spinacia oleracea
8.5
-
under reducing conditions Galdieria sulphuraria

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
the enzyme under oxidizing conditions or untreated is almost inactive with only a small increase starting at pH 9.0 and above Spinacia oleracea
additional information
-
the pH profile of the oxidized form of enzyme reveals an almost constant activity Galdieria sulphuraria