Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Spinacia oleracea |
expression in Escherichia coli, in this sequence of enzyme, the Cys that corresponds to Cys179 in the spinach enzyme is lacking | Galdieria sulphuraria |
Protein Variants | Comment | Organism |
---|---|---|
C155S | mutant is active | Spinacia oleracea |
C174S | mutant is active and can be inactivated by oxidation | Spinacia oleracea |
C179S | mutant is inactive | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, comparison of activities of wild-type and mutant enzymes in dependence of Mg2+ concentrations | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Galdieria sulphuraria | enzyme is a part of Calvin cycle | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-diphosphate + H2O | Spinacia oleracea | enzyme is a part of Calvin cycle | D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Brassica napus | - |
- |
- |
Galdieria sulphuraria | Q95AJ2 | red alga | - |
Glycine max | - |
- |
- |
Oryza sativa | - |
rice | - |
Pisum sativum | - |
pea | - |
Solanum tuberosum | - |
potato | - |
Spinacia oleracea | - |
- |
- |
Triticum aestivum | - |
wheat | - |
Purification (Comment) | Organism |
---|---|
- |
Spinacia oleracea |
- |
Galdieria sulphuraria |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | the chloroplast enzyme of higher plants can occur in an active form that is inactivated upon disulfide bridge formation between Cys155 and Cys174 | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of activities of oxidizied and reduced enzyme | Galdieria sulphuraria |
additional information | - |
comparison of activities of oxidizied and reduced wild-type and mutant enzymes | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Galdieria sulphuraria | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | enzyme is a part of Calvin cycle | Galdieria sulphuraria | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-diphosphate + H2O | enzyme is a part of Calvin cycle | Spinacia oleracea | D-fructose 6-phosphate + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
under reducing conditions | Spinacia oleracea |
8.5 | - |
under reducing conditions | Galdieria sulphuraria |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme under oxidizing conditions or untreated is almost inactive with only a small increase starting at pH 9.0 and above | Spinacia oleracea |
additional information | - |
the pH profile of the oxidized form of enzyme reveals an almost constant activity | Galdieria sulphuraria |