Protein Variants | Comment | Organism |
---|---|---|
R49C | less thermostable than wild type enzyme, wild type values for turnover number and Km-value | Sus scrofa |
R49D | less thermostable than wild type enzyme, wild type values for turnover number and Km-value, increased inhibition constant for fructose 2,6-diphosphate. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to competitive. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to uncompetitive. Loss of AMP cooperativity | Sus scrofa |
R49L | less thermostable than wild type enzyme, wild type values for turnover number and Km-value, increased inhibition constant for fructose 2,6-diphosphate. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to competitive. Loss of AMP cooperativity | Sus scrofa |
R49M | enzyme is more thermostable than wild type enzyme, kinetic properties are similar to the wild type enzyme. Loss of AMP cooperativity | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00344 | - |
fructose 1,6-diphosphate | mutant enzyme R49L | Sus scrofa | |
0.00351 | - |
fructose 1,6-diphosphate | wild type enzyme | Sus scrofa | |
0.00402 | - |
fructose 1,6-diphosphate | mutant enzyme K50M | Sus scrofa | |
0.00407 | - |
fructose 1,6-diphosphate | mutant enzyme R49C | Sus scrofa | |
0.00442 | - |
fructose 1,6-diphosphate | mutant enzyme R49D | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | - |
Sus scrofa | D-fructose 6-phosphate + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
fructose 1,6-diphosphate | wild type enzyme | Sus scrofa | |
20.1 | - |
fructose 1,6-diphosphate | mutant enzyme R49D | Sus scrofa | |
22.4 | - |
fructose 1,6-diphosphate | mutant enzyme K50M | Sus scrofa | |
23.6 | - |
fructose 1,6-diphosphate | mutant enzyme R49L and R49C | Sus scrofa |