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Literature summary for 3.1.3.11 extracted from
- Molecular characterization and resistance to hydrogen peroxide of two fructose-1,6-bisphosphatases from Synechococcus PCC 7942 (1996), Arch. Biochem. Biophys., 334, 27-36.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isoenzyme F-I and F-II, expression in Escherichia coli | Synechococcus sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | isoenzyme F-I is inhibited, isoenzyme F-II is not inhibited | Synechococcus sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
fructose 1,6-diphosphate | isoenzyme F-II | Synechococcus sp. | |
| 0.052 | - |
fructose 1,6-diphosphate | isoenzyme F-I | Synechococcus sp. | |
| 0.118 | - |
sedoheptulose-1,7-diphosphate | - |
Synechococcus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
4 * 37000, isoenzyme F-II, SDS-PAGE | Synechococcus sp. |
| 40000 | - |
4 * 40000, isoenzyme F-I, SDS-PAGE | Synechococcus sp. |
| 150000 | - |
isoenzyme F-II, gel filtration | Synechococcus sp. |
| 160000 | - |
isoenzyme F-I | Synechococcus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus sp. | - |
PCC 7942 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes: F-I and F-II | Synechococcus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.7 | - |
- |
Synechococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Synechococcus sp. | D-fructose 6-phosphate + phosphate | - |
? | |
| sedoheptulose 1,7-bisphosphate + H2O | activity with isoenzyme F-1, no activity with isoenzyme F-II | Synechococcus sp. | sedoheptulose 7-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 37000, isoenzyme F-II, SDS-PAGE | Synechococcus sp. |
| tetramer | 4 * 40000, isoenzyme F-I, SDS-PAGE | Synechococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
isoenzyme F-I | Synechococcus sp. |
| 9 | - |
isoenzyme F-II | Synechococcus sp. |
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