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Literature summary for 3.1.3.11 extracted from
- Fructose 1,6-diphosphatase from Rhodopseudomonas palustris. I. Purification and properties (1972), Arch. Biochem. Biophys., 152, 1-12.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cys | stimulates | Rhodopseudomonas palustris |  |
| DTT | activates | Rhodopseudomonas palustris | |
| GSH | activates | Rhodopseudomonas palustris | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Rhodopseudomonas palustris | |
| 8-hydroxyquinoline | - |
Rhodopseudomonas palustris | |
| Cs+ | - |
Rhodopseudomonas palustris | |
| EDTA | 0.1 mM, complete inhibition | Rhodopseudomonas palustris | |
| K+ | - |
Rhodopseudomonas palustris | |
| KCN | - |
Rhodopseudomonas palustris | |
| Li+ | - |
Rhodopseudomonas palustris | |
| Na+ | - |
Rhodopseudomonas palustris | |
| NH4+ | - |
Rhodopseudomonas palustris | |
| p-hydroxymercuribenzoate | - |
Rhodopseudomonas palustris | |
| sedoheptulose 1,7-diphosphate | competitive inhibition of activity with fructose 1,6-diphosphate | Rhodopseudomonas palustris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
fructose 1,6-diphosphate | - |
Rhodopseudomonas palustris | |
| 0.3 | - |
sedoheptulose 1,7-diphosphate | - |
Rhodopseudomonas palustris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | only 27% of the maximal activity observed with Mn2+ | Rhodopseudomonas palustris | |
| Mg2+ | Km: 3.3 mM | Rhodopseudomonas palustris | |
| Mg2+ | divalent cation required. Mn2+ or Mg2+ | Rhodopseudomonas palustris | |
| Mn2+ | divalent cation required. Mn2+ or Mg2+ | Rhodopseudomonas palustris | |
| Mn2+ | Km: 0.022 mM | Rhodopseudomonas palustris | |
| Mn2+ | Km: 0.078 mM | Rhodopseudomonas palustris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
sucrose density gradient centrifugation | Rhodopseudomonas palustris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodopseudomonas palustris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodopseudomonas palustris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.81 | - |
- |
Rhodopseudomonas palustris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.1 mM MnCl2, 1 mM DTT, 0.01 M Tris-HCl buffer, pH 7.4, stable for at least 3 months | Rhodopseudomonas palustris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Rhodopseudomonas palustris | D-fructose 6-phosphate + phosphate | - |
? | |
| sedoheptulose 1,7-bisphosphate + H2O | at 22.4% of the activity with fructose 1,6-diphosphate | Rhodopseudomonas palustris | sedoheptulose 7-phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 min, 65% loss of activity without Mn2+, 12% loss of activity in presence of 0.3 mM Mn2+ | Rhodopseudomonas palustris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate | Rhodopseudomonas palustris |
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