Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, stimulates | Rhodopseudomonas palustris | |
Cys | 1 mM, stimulates | Rhodopseudomonas palustris | |
dithiothreitol | 1 mM stimulates | Rhodopseudomonas palustris | |
EDTA | - |
Rhodopseudomonas palustris | |
sulfhydryl reagents | 1 mM, stimulate in order of decreasing stimulation activity: glutathione, cysteine, dithiothreitol, 2-mercaptoethanol | Rhodopseudomonas palustris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | no inhibition | Rhodopseudomonas palustris | |
p-hydroxymercuribenzoate | strong | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
sedoheptulose 1,7-diphosphate | - |
Rhodopseudomonas palustris | |
0.045 | - |
fructose 1,6-diphosphate | - |
Rhodopseudomonas palustris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km: 3.3 mM | Rhodopseudomonas palustris | |
Mg2+ | divalent cation required. Mn2+ or Mg2+ | Rhodopseudomonas palustris | |
Mn2+ | divalent cation required. Mn2+ or Mg2+ | Rhodopseudomonas palustris | |
Mn2+ | Km: 0.078 mM | Rhodopseudomonas palustris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
sucrose density gradient centrifugation | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | Rhodopseudomonas palustris | the enzyme may be involved in the regulation of the Calvin-Bassham carbon reduction cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodopseudomonas palustris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodopseudomonas palustris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Rhodopseudomonas palustris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodopseudomonas palustris |
Storage Stability | Organism |
---|---|
-20°C, 0.1 mM MnCl2, 1 mM DTT, 0.01 M Tris-HCl buffer, pH 7.4, stable for at least 3 months | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-diphosphate + H2O | the enzyme may be involved in the regulation of the Calvin-Bassham carbon reduction cycle | Rhodopseudomonas palustris | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | - |
Rhodopseudomonas palustris | D-fructose 6-phosphate + phosphate | - |
? | |
sedoheptulose 1,7-bisphosphate + H2O | at 22.4% of the activity with fructose 1,6-diphosphate | Rhodopseudomonas palustris | sedoheptulose 7-phosphate + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rhodopseudomonas palustris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | pH 7.5: 65% of maximal activity, pH 8.5: optimum | Rhodopseudomonas palustris |