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Literature summary for 3.1.3.11 extracted from
- Characteristics of fructose-1,6-bisphosphatase from the methanol-ultilizing yeast Hansenula polymorpha (1982), J. Gen. Microbiol., 128, 2313-2317.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | stimulates | Ogataea angusta |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Ogataea angusta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
fructose 1,6-diphosphate | enzyme from ethanol-grown cells | Ogataea angusta | |
| 0.029 | - |
fructose 1,6-diphosphate | enzyme from methanol-grown cells | Ogataea angusta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, enzyme from methanol-grown cells | Ogataea angusta | |
| Mn2+ | can substitute for Mg2+ with 50% efficiency, enzyme from methanol-grown cells | Ogataea angusta | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Ogataea angusta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the enzyme purified from ethanol-grown cells resembles that from methanol-grown cells in all properties investigated | Ogataea angusta | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, half-life: 1 week, enzyme from methanol-grown cells | Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Ogataea angusta | D-fructose 6-phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: 1 h, enzyme from methanol-grown cells | Ogataea angusta |
| 37 | - |
half-life: 5 min, enzyme from methanol-grown cells | Ogataea angusta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
enzyme from methanol-grown cells | Ogataea angusta |
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