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Literature summary for 3.1.3.11 extracted from
- Fructose-1,6-diphosphatase and sedoheptulose-1,7-diphosphatase from Candida utilis (1975), Methods Enzymol., 42C, 347-353.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CoA | activates | Cyberlindnera jadinii |  |
| cystamine | activates | Cyberlindnera jadinii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Cyberlindnera jadinii | |
| fructose 1,6-diphosphate | above 0.1 mM | Cyberlindnera jadinii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
fructose 1,6-diphosphate | - |
Cyberlindnera jadinii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required. Mn2+ or Mg2+ | Cyberlindnera jadinii | |
| Mn2+ | divalent cation required. Mn2+ or Mg2+ | Cyberlindnera jadinii | |
| Mn2+ | less active in activation than Mg2+ | Cyberlindnera jadinii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
4 * 35000, SDS-PAGE | Cyberlindnera jadinii |
| 130000 | - |
sucrose density gradient centrifugation | Cyberlindnera jadinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 73 | - |
- |
Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Cyberlindnera jadinii | D-fructose 6-phosphate + phosphate | - |
? | |
| sedoheptulose 1,7-bisphosphate + H2O | no activity | Cyberlindnera jadinii | sedoheptulose 7-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000, SDS-PAGE | Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | 8.8 | - |
Cyberlindnera jadinii |
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Release 2023.1 (January 2023)
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