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Literature summary for 3.1.3.105 extracted from

  • Borisova, M.; Gisin, J.; Mayer, C.
    The N-acetylmuramic acid 6-phosphate phosphatase MupP completes the Pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance (2017), mBio, 8, e00092 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.105

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 N-acetyl-D-muramate 6-phosphate pH 7.6, 22°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ very low activity in absence of Mg2+, 16fold increase in activity in presence of 10 mM Mg2+ Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q88M11
-
-
Pseudomonas putida DSM 6125 Q88M11
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate Pseudomonas putida ?
-
 ?
additional information no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate Pseudomonas putida DSM 6125 ?
-
 ?
N-acetyl-D-muramate 6-phosphate + H2O
-
 Pseudomonas putida N-acetyl-D-muramate + phosphate
-
 ?
N-acetyl-D-muramate 6-phosphate + H2O
-
 Pseudomonas putida DSM 6125 N-acetyl-D-muramate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 25890, calculated, recombinant His-tagged protein Pseudomonas putida

Synonyms

Synonyms Comment Organism
mupP
-
 Pseudomonas putida
N-acetylmuramic acid 6-phosphate phosphatase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 40 min, 10% loss of activity Pseudomonas putida
37
-
 40 min, 70% loss of activity Pseudomonas putida
45
-
 40 min, complete inactivation Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.88
-
 N-acetyl-D-muramate 6-phosphate pH 7.6, 22°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Pseudomonas putida

General Information

General Information Comment Organism
physiological function enzyme is part of the anabolic recycling pathway of Pseudomonas species and specifically converts MurNAc 6-phosphate to MurNAc. A MupP mutant is highly susceptible to fosfomycin, accumulates large amounts of MurNAc 6-phosphate, and shows lower levels of UDP-MurNAc than wild-type cells Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.84
-
 N-acetyl-D-muramate 6-phosphate pH 7.6, 22°C Pseudomonas putida