BRENDA - Enzyme Database
show all sequences of 3.1.3.103

Structure-function analysis of 2-keto-3-deoxy-D-glycero-D-galactonononate-9-phosphate phosphatase defines specificity elements in type C0 haloalkanoate dehalogenase family members

Lu, Z.; Wang, L.; Dunaway-Mariano, D.; Allen, K.N.; J. Biol. Chem. 284, 1224-1233 (2009)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
structures of BT_1713 liganded to the cofactor Mg2+ and complexed with tungstate or VO3-/Neu5Ac, to 1.1, 1.85, and 1.63 A resolution, respectively. The active site lies at the subunit interface and Arg64, Lys67, and Glu56 are the key residues involved in sugar binding essential for catalytic function
Bacteroides thetaiotaomicron
Engineering
Amino acid exchange
Commentary
Organism
E56A
200fold decrease in catalytic efficiency
Bacteroides thetaiotaomicron
R64A
loss of catalytic activity
Bacteroides thetaiotaomicron
S37A
2fold decrease in catalytic efficiency
Bacteroides thetaiotaomicron
T34A
20fold decrease in catalytic efficiency
Bacteroides thetaiotaomicron
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
wild-type, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.18
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant T34A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.2
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant S37A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.41
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant E56A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacteroides thetaiotaomicron
Q8A712
-
-
Bacteroides thetaiotaomicron DSM 2079
Q8A712
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
738574
Bacteroides thetaiotaomicron
3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
-
-
?
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
738574
Bacteroides thetaiotaomicron DSM 2079
3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.026
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant E56A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.09
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant T34A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
1.2
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant S37A, pH 7.0, 37°C; wild-type, pH 7.0, 37°C
Bacteroides thetaiotaomicron
Crystallization (Commentary) (protein specific)
Crystallization
Organism
structures of BT_1713 liganded to the cofactor Mg2+ and complexed with tungstate or VO3-/Neu5Ac, to 1.1, 1.85, and 1.63 A resolution, respectively. The active site lies at the subunit interface and Arg64, Lys67, and Glu56 are the key residues involved in sugar binding essential for catalytic function
Bacteroides thetaiotaomicron
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E56A
200fold decrease in catalytic efficiency
Bacteroides thetaiotaomicron
R64A
loss of catalytic activity
Bacteroides thetaiotaomicron
S37A
2fold decrease in catalytic efficiency
Bacteroides thetaiotaomicron
T34A
20fold decrease in catalytic efficiency
Bacteroides thetaiotaomicron
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
wild-type, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.18
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant T34A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.2
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant S37A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.41
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant E56A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
738574
Bacteroides thetaiotaomicron
3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
-
-
?
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
738574
Bacteroides thetaiotaomicron DSM 2079
3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.026
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant E56A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.09
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant T34A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
1.2
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant S37A, pH 7.0, 37°C; wild-type, pH 7.0, 37°C
Bacteroides thetaiotaomicron
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.06
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant E56A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.5
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant T34A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
6
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant S37A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
12
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
wild-type, pH 7.0, 37°C
Bacteroides thetaiotaomicron
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.06
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant E56A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
0.5
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant T34A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
6
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
mutant S37A, pH 7.0, 37°C
Bacteroides thetaiotaomicron
12
-
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
wild-type, pH 7.0, 37°C
Bacteroides thetaiotaomicron
Other publictions for EC 3.1.3.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738574
Lu
Structure-function analysis of ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079
J. Biol. Chem.
284
1224-1233
2009
-
-
-
1
4
-
-
4
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
4
4
738039
Wang
Human symbiont Bacteroides the ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079
Chem. Biol.
15
893-897
2008
-
-
-
-
-
-
-
3
-
-
-
2
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-