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Literature summary for 3.1.3.102 extracted from

  • Rawat, R.; Sandoval, F.J.; Wei, Z.; Winkler, R.; Roje, S.
    An FMN hydrolase of the haloacid dehalogenase superfamily is active in plant chloroplasts (2011), J. Biol. Chem., 286, 42091-42098.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.102

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0142
-
 FMN pH 8.0, 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Pisum sativum cv. Bohatyr 9507
-
plastid
-
 Arabidopsis thaliana 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana
Mg2+ required Pisum sativum cv. Bohatyr

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59400
-
 gel filtration Pisum sativum cv. Bohatyr

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMN + H2O Arabidopsis thaliana
-
 riboflavin + phosphate
-
 ?
FMN + H2O Pisum sativum cv. Bohatyr
-
 riboflavin + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4HQA8
-
-
Pisum sativum cv. Bohatyr
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana
-
 Pisum sativum cv. Bohatyr

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00752
-
 pH 8.0, 30°C Pisum sativum cv. Bohatyr
1.1
-
 pH 8.0, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + H2O
-
 Arabidopsis thaliana riboflavin + phosphate
-
 ?
FMN + H2O
-
 Pisum sativum cv. Bohatyr riboflavin + phosphate
-
 ?
FMN + H2O high specificity for FMN. The enzyme does not hydrolyze the following substrates: ADP, AMP, ATP, CTP, GTP, IMP, UTP, xanthosine 5'-monophosphate, dATP, glucose 1-phosphate, glucose 6-phosphate, glucose 1,6-bisphosphate, and fructose 1,6-bisphosphate, pyridoxal 5'-phosphate and NADP+ Arabidopsis thaliana riboflavin + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 25000-30000, SDs-PAGE Pisum sativum cv. Bohatyr

Synonyms

Synonyms Comment Organism
AtcpFHy1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana
30
-
 assay at Pisum sativum cv. Bohatyr
40
-
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 65% of maximal activity, 50°C: about 55% of maximal activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.59
-
 FMN pH 8.0, 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Arabidopsis thaliana
8
-
 assay at Arabidopsis thaliana
8
-
 assay at Pisum sativum cv. Bohatyr

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: about 90% of maximal activity, pH 8.5: about 45% of maximal activity Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
41.5
-
 FMN pH 8.0, 30°C Arabidopsis thaliana