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Literature summary for 3.1.3.10 extracted from

  • Kim, Y.; Park, I.; Nam, B.; Kong, H.; Kim, W.; Lee, S.; Kim, K.
    Gene cloning, expression, and characterization of glucose-1-phosphatase from Enterobacter cloacae B11 (2010), Fish. Aqua. Sci., 13, 49-55.
No PubMed abstract available
Search for more literature for 3.1.3.10

Cloned(Commentary)

Cloned (Comment) Organism
gene AgpEnB11, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Enterobacter cloacae

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Enterobacter cloacae 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Enterobacter cloacae
Co2+ activates Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, recombinant His-tagged enzyme, SDS-PAGE Enterobacter cloacae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate + H2O Enterobacter cloacae high activity D-glucose + phosphate
-
 ?
alpha-D-glucose 1-phosphate + H2O Enterobacter cloacae B11 high activity D-glucose + phosphate
-
 ?
alpha-D-glucose 6-phosphate + H2O Enterobacter cloacae high activity D-glucose + phosphate
-
 ?
alpha-D-glucose 6-phosphate + H2O Enterobacter cloacae B11 high activity D-glucose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
 isolate from seawater, gene AgpEnB11
-
Enterobacter cloacae B11
-
 isolate from seawater, gene AgpEnB11
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + H2O high activity Enterobacter cloacae D-glucose + phosphate
-
 ?
alpha-D-glucose 1-phosphate + H2O high activity Enterobacter cloacae B11 D-glucose + phosphate
-
 ?
alpha-D-glucose 6-phosphate + H2O high activity Enterobacter cloacae D-glucose + phosphate
-
 ?
alpha-D-glucose 6-phosphate + H2O high activity Enterobacter cloacae B11 D-glucose + phosphate
-
 ?
additional information the enzyme hydrolyzes a wide variety of phosphorylated compounds, primarily small monosaccharide phosphates, overview Enterobacter cloacae ?
-
 ?
additional information the enzyme hydrolyzes a wide variety of phosphorylated compounds, primarily small monosaccharide phosphates, overview Enterobacter cloacae B11 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, recombinant His-tagged enzyme, SDS-PAGE Enterobacter cloacae

Synonyms

Synonyms Comment Organism
acid glucose-1-phosphatase
-
 Enterobacter cloacae
AGP
-
 Enterobacter cloacae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant His-tagged enzyme Enterobacter cloacae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity profile, overview Enterobacter cloacae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 stability profile, overview Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant His-tagged enzyme Enterobacter cloacae

pH Range

pH Minimum pH Maximum Comment Organism
2 10 activity profile, overview Enterobacter cloacae

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10 stability profile, overview Enterobacter cloacae