Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.10 extracted from

  • Kim, Y.O.; Kim, H.W.; Park, I.S.; Lee, J.H.; Lee, S.J.; Kim, K.K.
    Purification, characterization, and gene cloning of glucose-1-phosphatase from Citrobacter braakii (2009), J. Gen. Appl. Microbiol., 55, 345-350.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
D-glucose 6-phosphate Vmax: 0.8 micromol/min/mg Citrobacter braakii
1.9
-
D-glucose 6-phosphate Vmax: 2.2 micromol/min/mg Citrobacter braakii
5.12
-
D-glucose 1-phosphate Vmax: 1.9 micromol/min/mg Citrobacter braakii
9
-
D-mannose 6-phosphate Vmax: 2.6 micromol/min/mg Citrobacter braakii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43710
-
calculated from cDNA Citrobacter braakii
46000
-
SDS-PAGE Citrobacter braakii

Organism

Organism UniProt Comment Textmining
Citrobacter braakii D3GGN3
-
-

Purification (Commentary)

Purification (Comment) Organism
(NH4)2SO4 precipitation, DEAE-sepharose chromatography, CM-sepharose chromatography and FPLC chromatography Citrobacter braakii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + H2O
-
Citrobacter braakii D-fructose + phosphate
-
?
D-glucose 1-phosphate + H2O
-
Citrobacter braakii D-glucose + phosphate
-
?
D-glucose 6-phosphate + H2O
-
Citrobacter braakii D-glucose + phosphate
-
?
D-mannose 6-phosphate + H2O
-
Citrobacter braakii D-mannose + phosphate
-
?

Synonyms

Synonyms Comment Organism
agpC
-
Citrobacter braakii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at and optimal temperature Citrobacter braakii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
enzyme ist stable up to 55°C 30 min Citrobacter braakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at and optimal pH Citrobacter braakii

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7.5
-
Citrobacter braakii