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Literature summary for 3.1.3.1 extracted from
- Purification and characterization of an extracellular alkaline phosphatase from Penicillium chrysogenum (1996), Prep. Biochem. Biotechnol., 26, 171-181.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | weak inhibition | Penicillium chrysogenum |  |
| EDTA | - |
Penicillium chrysogenum | |
| Hg2+ | - |
Penicillium chrysogenum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
p-nitrophenyl phosphate | - |
Penicillium chrysogenum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium chrysogenum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | enhances activity to a lesser extent than Mg2+ | Penicillium chrysogenum | |
| Zn2+ | enhances activity to a lesser extent than Mg2+ | Penicillium chrysogenum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
1 * 58000, SDS-PAGE | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | contains 6-11% carbohydrate per weight | Penicillium chrysogenum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium chrysogenum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-naphthyl phosphate + H2O | - |
Penicillium chrysogenum | 1-naphthol + phosphate | - |
? | |
| etoposide phosphate + H2O | - |
Penicillium chrysogenum | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Penicillium chrysogenum | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 58000, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
Penicillium chrysogenum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 35% of maximal activity, 60°C: about 30% of maximal activity | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
hydrolysis of p-nitrophenyl phosphate | Penicillium chrysogenum |
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