KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
p-nitrophenyl phosphate | - |
Thermotoga neapolitana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enhances activity, twice as active than with either Zn2+ or Mn2+ | Thermotoga neapolitana | |
Mn2+ | enhances activity | Thermotoga neapolitana | |
Zn2+ | enhances activity | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Thermotoga neapolitana |
87000 | - |
gel filtration | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga neapolitana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
663.3 | - |
- |
Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-ADP + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
5'-ATP + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
D-fructose 6-phosphate + H2O | - |
Thermotoga neapolitana | D-fructose + phosphate | - |
? | |
fructose 1,6-diphosphate + H2O | - |
Thermotoga neapolitana | ? | - |
? | |
glucose 1-phosphate + H2O | - |
Thermotoga neapolitana | glucose + phosphate | - |
? | |
Glucose 6-phosphate + H2O | - |
Thermotoga neapolitana | Glucose + phosphate | - |
? | |
glycerophosphate + H2O | - |
Thermotoga neapolitana | glycerol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Thermotoga neapolitana | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga neapolitana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 95 | 60°C: about 45% of maximal activity, 9°C: about 60% of maximal activity | Thermotoga neapolitana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability is increased 30fold by Co2+ and 23fold by Mg2+ | Thermotoga neapolitana |
90 | - |
half-life: 238 min, in presence of Co2+ | Thermotoga neapolitana |
100 | - |
40 min, in presence of 40 mM Co2+, 3% loss of activity | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.9 | - |
- |
Thermotoga neapolitana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11.5 | pH 8.0: about 40% of maximal activity, pH 11.5: about 45% of maximal activity | Thermotoga neapolitana |