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Literature summary for 3.1.3.1 extracted from

  • Mori, S.; Okamoto, M.; Nishibori, M.; Ichimura, M.; Sakiyama, J.; Endo, H.
    Purification and characterization of alkaline phosphatase from Bacillus stearothermophilus (1999), Biotechnol. Appl. Biochem., 29, 235-239.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.114
-
p-nitrophenyl phosphate
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ bivalent metal ions required, maximal enhancement in presence of Mg2+ Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.43
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
Geobacillus stearothermophilus p-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
1 h, 25% loss of activity, 12 h, 62% loss of activity Geobacillus stearothermophilus
60
-
about 65% loss of activity after 1 h, complete loss of activity after 6 h Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7.0: about 10% of maximal activity, pH 10.5: about 35% of maximal activity Geobacillus stearothermophilus