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Literature summary for 3.1.3.1 extracted from

  • Nomoto, M.; Ohsawa, M.; Wang, H.L.; Chen, C.C.; Yeh, K.W.
    Purification and characterization of extracellular alkaline phosphatase from an alkalophilic bacterium (1988), Agric. Biol. Chem., 52, 1643-1647.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 83% inhibition Bacillus sp. OK-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
4-nitrophenyl phosphate
-
Bacillus sp. OK-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Bacillus sp. OK-1
110000
-
gel filtration Bacillus sp. OK-1

Organism

Organism UniProt Comment Textmining
Bacillus sp. OK-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. OK-1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus sp. OK-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bacillus sp. OK-1 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Bacillus sp. OK-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. OK-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable up to Bacillus sp. OK-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
Bacillus sp. OK-1

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
3 h, about 40% loss of activity Bacillus sp. OK-1
11.5
-
3 h, about 50% loss of activity Bacillus sp. OK-1