Literature summary for 3.1.3.1 extracted from
Nguyen, H.; Wu, S.; Su, C.; Hwang, T.
Biochemical, biophysical, and thermal properties of alkaline phosphatase from thermophile Thermus sp. NTU-237 (2017), Biotechnol. Agron. Soc. Environ., 21, 117-126 .
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
NaCl |
the highest enzyme activity is observed when 100 mM NaCl is added to the assay reaction |
Thermus sp. NTU-237 |
|
Tris-HCl |
- |
Thermus sp. NTU-237 |
|
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Escherichia coli |
Thermus sp. NTU-237 |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
glycerol |
complete inhibition at 30% (v/v) |
Thermus sp. NTU-237 |
|
SDS |
complete inhibition at 5% (w/v) |
Thermus sp. NTU-237 |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.081 |
- |
4-nitrophenyl phosphate |
at pH 10.5 and 70°C |
Thermus sp. NTU-237 |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
109000 |
- |
gel filtration |
Thermus sp. NTU-237 |
Organism
Organism |
UniProt |
Comment |
Textmining |
Thermus sp. NTU-237 |
A0A1B2YKT4 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
Ni-NTA column chromatography, and Sepharose 6 gel filtration |
Thermus sp. NTU-237 |
Storage Stability
Storage Stability |
Organism |
25°C, 0.1 M Tris-HCl buffer (pH 10.5), 30 days, 68% loss of activity |
Thermus sp. NTU-237 |
37°C, 0.1 M Tris-HCl buffer (pH 10.5), 30 days, 70% loss of activity |
Thermus sp. NTU-237 |
4°C, 0.1 M Tris-HCl buffer (pH 10.5), 30 days, 58% loss of activity |
Thermus sp. NTU-237 |
4°C, 25°C or 37°C, 0.1 M Tris-HCl buffer (pH 10.5), 15 days, 50% loss of activity |
Thermus sp. NTU-237 |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
4-nitrophenyl phosphate + H2O |
- |
Thermus sp. NTU-237 |
4-nitrophenol + phosphate |
- |
? |
|
5-bromo-4-chloro-3-indolyl phosphate + H2O |
- |
Thermus sp. NTU-237 |
5-bromo-4-chloro-indole + phosphate |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
homodimer |
2 * 54000, SDS-PAGE |
Thermus sp. NTU-237 |
Synonyms
Synonyms |
Comment |
Organism |
APase |
- |
Thermus sp. NTU-237 |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
80 |
- |
- |
Thermus sp. NTU-237 |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
70 |
100 |
at 70, 90 and 100°C, the enzyme retains more than 50% of its maximum activity at 80°C |
Thermus sp. NTU-237 |
Turnover Number [1/s]
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
6.08 |
- |
4-nitrophenyl phosphate |
at pH 10.5 and 70°C |
Thermus sp. NTU-237 |
|