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Literature summary for 3.1.3.1 extracted from

  • Nguyen, H.; Wu, S.; Su, C.; Hwang, T.
    Biochemical, biophysical, and thermal properties of alkaline phosphatase from thermophile Thermus sp. NTU-237 (2017), Biotechnol. Agron. Soc. Environ., 21, 117-126 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
NaCl the highest enzyme activity is observed when 100 mM NaCl is added to the assay reaction Thermus sp. NTU-237
Tris-HCl
-
Thermus sp. NTU-237

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermus sp. NTU-237

Inhibitors

Inhibitors Comment Organism Structure
glycerol complete inhibition at 30% (v/v) Thermus sp. NTU-237
SDS complete inhibition at 5% (w/v) Thermus sp. NTU-237

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
4-nitrophenyl phosphate at pH 10.5 and 70°C Thermus sp. NTU-237

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109000
-
gel filtration Thermus sp. NTU-237

Organism

Organism UniProt Comment Textmining
Thermus sp. NTU-237 A0A1B2YKT4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Sepharose 6 gel filtration Thermus sp. NTU-237

Storage Stability

Storage Stability Organism
25°C, 0.1 M Tris-HCl buffer (pH 10.5), 30 days, 68% loss of activity Thermus sp. NTU-237
37°C, 0.1 M Tris-HCl buffer (pH 10.5), 30 days, 70% loss of activity Thermus sp. NTU-237
4°C, 0.1 M Tris-HCl buffer (pH 10.5), 30 days, 58% loss of activity Thermus sp. NTU-237
4°C, 25°C or 37°C, 0.1 M Tris-HCl buffer (pH 10.5), 15 days, 50% loss of activity Thermus sp. NTU-237

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Thermus sp. NTU-237 4-nitrophenol + phosphate
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O
-
Thermus sp. NTU-237 5-bromo-4-chloro-indole + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 54000, SDS-PAGE Thermus sp. NTU-237

Synonyms

Synonyms Comment Organism
APase
-
Thermus sp. NTU-237

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermus sp. NTU-237

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 at 70, 90 and 100°C, the enzyme retains more than 50% of its maximum activity at 80°C Thermus sp. NTU-237

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.08
-
4-nitrophenyl phosphate at pH 10.5 and 70°C Thermus sp. NTU-237